SINGLE StEP Columns (patented) represent an innovative step forward in chromatography. SINGLE StEP Columns (patented) allow for a simple screw top column that can be used for liquid or dry loading by opening the cap and pouring in liquid or dropping in dry material. The columns are a universal fit for all different flash instruments. C18 columns replace prep chromatography and can be loaded 1:10 (10%). C18 columns are reusable up to 20 times.
C18 columns operate under reverse phase conditions, i.e., 100% Water → 100% Acetonitrile (reverse phase conditions with increasing solvent phase polarity). These columns replace Prep HPLC conditions. C18 columns are available in a variety of sizes ranging from 5.5grams to 355grams. They can be loaded up to 10% of stationary phase weight. Our C18 columns can be re-used up to 20 times! General guideline for compound loading is 10% of material weight.
We do not offer C18 TLC Plates to screen for profile matching, however, LCMS runs will give similar results. Like any reverse phase application, peak shapes can be optimized by addition of appropriate buffer or formic acid (increasing acidity). Please contact us for further assistance.
U.S. Patent 7,138,061, 7,381,327, 7,410,571 and other patents pending. U.K. Patent 2413972.