Protein Precipitation with SINGLE StEP Filter Vial™ Patent No. 7,790,117

Pipette 360μL of solvent: Acetonitrile, Methanol... into SINGLE StEP Filter shell Vial.

Add 40μL of sample to shell Via

Insert plunger and press 2/3 of the way down.

(sample should not be filtered at this point)

Vortex Sample

20-40 seconds

Protein Crash

Recommended PVDF .2μM Filter Vial

Use #35531-200 or 35531-500

Press plunger completely.

Filtered Sample

Precipitated Protein

Same Filter Vial
               Straight to
                    HPLC or LC/MS

Filter Vial
For Protein Precipitation

Protein Precipitation

Thomson Filter Vials save multiple steps and time by minimizing the handling of samples. Older methods, which use centrifugation, increase loss of samples by having multiple transfer steps.

The Perfect "Protein Crash" can be created by mixing plasma, with the correct ratio of an appropriate solvent, in a 6X-12X dilution.

Thomson Filter Vials can by used by adding different polar solvents to the plasma containing the compound of interest. Most people use Acetonitrile or Methanol, but other solvents can suffice. We have PVDF, PES, and Nylon membranes for performing a "Protein Crash." PVDF membranes seem to be the most popular with minimal protein or small molecule binding.

Thomson Filter Vials provide an optimized solution for your "Protein Crash."