AstraZeneca vs. Thomson Single Step Column

Patent 7,138,061, 7,381,327, 7,410,571, 8,070,957 and 8,066,875. U.K. Patent 2413972.

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Why?

Annual spend is considerable $↓
Make sure we are getting good value - vendors come and go
Product is fit for purpose

The Study

Standard compound mixture 2-Nitroaniline/4-Nitroaniline
Loading: 20:1 wt ratio Silica:mixture (dry loading)
Conditions: 25% EtOAcin Hexane with solvent reference (PhCH3)
Flow: 40 mL/min, detection @ 254nm
Determine % recovery (mass balance)
Repeat run (n = 3) to minimize instrument/operator error

Conclusion

Thomson SINGLE StEP (patented) simple loading & repeatable

Thomson Instrument (40g)

	% Recovery 2-nitroaniline	% Recovery 4-nitroaniline
Thomson - 001	quantitative	quantitative
Thomson - 002	99	97
Thomson - 003	quantitative	98

2-Nitroaniline (4 tubes)

4-Nitroaniline (17 tubes)

No TLC needed

Loading - direct

Time 21 min

BiotageSNAP (50g)*

	% Recovery 2-nitroaniline	% Recovery 4-nitroaniline
Biotage - 001	quantitative	83
Biotage - 002	quantitative	74
Biotage - 003	ND	ND

2-Nitroaniline (8 tubes)

4-Nitroaniline (20+ tubes)

TLC needed

Loading - SNAP Cartridge

Time 30+ min

* The amount of aniline mixture added to the Biotagecolumn was adjusted to a total of 2.5 g to reflect the larger mass of silicai (silica:compound ratio was maintained at 20:1 for each column tested).

RediSepRf(40g)

	% Recovery 2-nitroaniline	% Recovery 4-nitroaniline
ISCO Rf - 001	quantitative	92
ISCO Rf - 002	97	93
ISCO Rf - 003	quantitative	94

2-Nitroaniline (5 tubes)

4-Nitroaniline (20 tubes)

No TLC needed

Loading - sample cartridge

Time 25 min

RediSepRf(40g) Variability

Patent 7,138,061, 7,381,327, 7,410,571, 8,070,957 and 8,066,875. U.K. Patent 2413972.