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SINGLE StEP Flash Columns Patented
Easy to Use...
And a column for every need...
- Range from 4g-300g and are rated to 200 psi
- Simple Screw Top - Easily Liquid or Dry Load Directly on Columns
- Works on Biotage®, Isco®, and SINGLE StEP Pumps
- Available in many choices of Normal and Reversed Phase Sorbents
- Band can be seen by Color or UV on the Column
- High Resolution Packing
- Direct Replacement for Glass Columns
SINGLE StEP Flash Columns
Flash Columns
The SINGLE StEP Columns™ are versatile enough to be used on any machine. SINGLE StEP™ columns are clear, so that one may see their bands or use a UV light to see their bands when using it in the hood (Darkened Hood). The SINGLE StEP™ columns have luer fittings which allow for attachment to any machine including: Biotage©, Isco®, SINGLE StEP© Pump units, etc. SINGLE StEP™ columns fit into the slots of Isco® Flash Machines. SINGLE StEP columns fit onto all Biotage Horizons™, manual, SP-1™ and Isolera® by luer fittings, eliminating the need for compression fittings. SINGLE StEP™ columns can be easily pre-equilibrated. SINGLE StEP™ columns have an added advantage that they allow direct injection as close to the sorbent bed as possible, either by liquid or dry loading, directly to the column. SINGLE StEP™ Columns are easy to use whether you have expensive equipment or you are just starting a lab.
| Column-Merck Silica | Compound Loading | Flow Rates | CV |
| 4913102 SINGLE StEP 4g 20/case | 50mg-200mg | 12mL/minute | 8mL |
| 4913109 SINGLE StEP 12g 20/case | 300mg-600mg | 18mL/minute | 24mL |
| 4913144 SINGLE StEP 25g 20/case | 700mg-1.4g | 25mL/minute | 50mL |
| 4913119 SINGLE StEP 40g 20/case | 1.5g-2.5g | 40mL/minute | 80mL |
| 4913122 SINGLE StEP 80g 20/case | 2.6g-4.1g | 55mL/minute | 160mL |
| 4913124 SINGLE StEP 90g 20/case | 4.2g-5.6g | 55mL/minute | 180mL |
| 4913126 SINGLE StEP 110g 12/case | 5.8g-6.5g | 55mL/minute | 220mL |
| 4913128 SINGLE StEP 160g 12/case | 7.2g-8.4g | 65mL/minute | 320mL |
| 4913130 SINGLE StEP 240g 10/case | 11.5g-13.8g | 85mL/minute | 480mL |
| 4913134 SINGLE StEP 300g 8/case | 14.4g-17.0g | 95mL/minute | 600mL |
| Column | Compound Loading | Flow Rates | CV |
| 574200 SINGLE StEP 4g 20/case | 50mg-150mg | 12mL/minute | 8mL |
| 574204 SINGLE StEP 12g 20/case | 200mg-500mg | 18mL/minute | 24mL |
| 574206 SINGLE StEP 25g 20/case | 700mg-1.4g | 25mL/minute | 50mL |
| 574208 SINGLE StEP 40g 20/case | 1.3g-2.0g | 40mL/minute | 80mL |
| 574212 SINGLE StEP 80g 20/case | 2.3g-3.9g | 55mL/minute | 160mL |
| 574216 SINGLE StEP 90g 20/case | 4.1g-5.2g | 55mL/minute | 180mL |
| 574218 SINGLE StEP 110g 12/case | 5.8g-6.5g | 55mL/minute | 220mL |
| 574220 SINGLE StEP 160g 12/case | 7.2g-8.4g | 65mL/minute | 320mL |
| 574222 SINGLE StEP 240g 10/case | 11.5g-13.8g | 85mL/minute | 480mL |
| 574224 SINGLE StEP 300g 8/case | 14.4g-17.0g | 95mL/minute | 600mL |
| Sizes for each column | ||
| 491250 | Head Space Frits for 4g | 100/case |
| 491252 | Head Space Frits for 12g | 100/case |
| 491253 | Head Space Frits for 25g | 100/case |
| 491254 | Head Space Frits for 40g | 100/case |
| 491256 | Head Space Frits for 80g or 90g | 100/case |
| 491258 | Head Space Frits for 110g or 160g | 100/case |
| 491260 | Head Space Frits for 240g or 300g | 100/case |
