Thomson Well Plates

Thomson’s well plates represent a cross section of plates for various types of assays, cell growth, and storage. We have the option in 96 and 24-well formats for scientists’ needs. Thomson Instrument Company additionally offers a variety of capmats, lids, and seals for covering your culture plates.

Although we carry a catalog of plates in our inventory, here are our most popular for insect and mammalian growth with our recommended protocol. To see our entire selection of well plates, see our Well Plates page.


96 Well Screening Protocol for Mammalian/Insect Cells

Materials

  • 96 Well Plate, 2mL, Square Well, Round Bottom, Individually Wrapped with Lid | Sterile: p/n 931134

Methods

  1. Maintain cell stocks in appropriate growth medium. Split cultures the day before transfection to an appropriate density to ensure log phase growth at the time of transfection.
  2. Seed cells at 500uL/well. The optimal seeding density will depend on the cell line, please use cell line recommended density.
  3. Transfect cells according to established transfection protocol. Scale transfection reagent/DNA/feeds on a volumetric basis from what is used for larger scale cultures.
  4. Seal plates with plastic lids or airporous seals and transfer to shaker overnight at 800rpm and 37°C.
  5. Harvest cells at the time point established for larger scale cultures. Pellet cells by centrifugation at 2500g for 20min at 4°C.
  6. Reserve either the culture media or the pellet depending on the application and proceed to downstream processing.

Notes

  • The most critical factor in cell viability is aeration. Optimal results will be achieved using shakers with 3mm orbit diameters. We do not recommend working in 96 well formation using shakers with standard 25mm throws.
  • Thomson filter plates are a great complimentary product for downstream purification applications.
    • 96 Well Filter Plate, 2mL, Long Drip | 25µm Polypropylene: p/n 931919
  • Maximum suggested centrifugation: 3000g.

To see our entire selection of well plates, see our Well Plates page

96 Well Screening Protocol Part Numbers

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24 Well Screening Protocol for Mammalian/Insect Cells

Materials

  • 24 Well Plate, 10.4mL, Square Well, Round Bottom, Individually Wrapped with Lid | Sterile: p/n 931568
  • 24 Well Plate, 10.8mL, Square Well, Pyramid Bottom, Individually Wrapped with Lid | Sterile: p/n 931571

Methods

  1. Maintain cell stocks in appropriate growth media. Split cultures the day before transfection to an appropriate density to ensure log phase growth at the time of transfection.
  2. Seed cells at 4-5mL/well. The optimal seeding density will depend on the cell line, please use cell line recommended density.
  3. Transfect cells according to established transfection protocol. Scale transfection reagent/DNA/feeds on a volumetric basis from what is used for larger scale cultures.
  4. Cover plates with plastic lids and transfer to shaker overnight at 350rpm and 37°C.
  5. Harvest cells at the time point established for larger scale cultures. Pellet cells by centrifugation at 2500g for 20min at 4°C.
  6. Reserve either the culture media or the pellet depending on the application and proceed to downstream processing.

Notes

  • The most critical factor in cell viability is aeration. Optimal results will be achieved using shakers with 12.5mm, or even 3mm orbit diameters. Cultures grown on shakers with standard 25mm throws, will likely need increased rotational speed or decreased culture volume.

To see our entire selection of well plates, see our Well Plates page

24 Well Screening Protocol Part Numbers

click a product to view more information

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