ASMS 2015 - Improved Sample Prep of Meat & Fish for Contaminate Analysis by LC/MS using Thomson eXtractor3D|FV™

Poster presented at ASMS 2015
Multiple papers used:
Multiclass multiresidue analysis of >100 veterinary drug residues in bovine tissues by filter-vial dispersive-SPE & LC-MS/MS

Comparison of different methods of extraction for incurred contaminants in fish using the eXtractor3D|FV® & analyzed by LPGC-MS/MS

High-throughput analysis is needed to meet demand for monitoring of veterinary drug residues in food animal tissues. The current veterinary drug residue monitoring method used by the USDA Food Safety and Inspection Service (FSIS) uses a combination of hexane-partitioning, dispersive-SPE, and solvent evaporation to achieve adequate cleanup for 20 mg equivalent sample injections to meet regulatory detection limit needs. This extra effort adds to the time and cost of the method and limits sample throughput. An improved method is developed and validated to streamline the sample preparation and LC-MS/MS method for identification and quantification of >120 veterinary drugs in bovine animal tissues for use in high throughput monitoring in the FSIS National Residue Program.

The goal of this study was to investigate variables impacting extraction yields of incurred pesticides and environmental contaminants: polycyclic aromatic hydrocarbons (PAHs), polychlorinated biphenyls (PCBs), polybrominated diphenyl ethers (PBDEs), and flame retardants (FRs) in fish samples of white croaker and salmon. We sought to compare extraction efficiencies of different shakers and extraction devices including the determination of the optimum shaking/extraction time, sample size, and sample-to-solvent ratio. In Filter-Vial Dispersive Solid-Phase Extraction with MgSO4, Z-Sep, C18, and primary secondary amine sorbents were used for sample clean-up in the Thomson eXtractor3D|FV®. Samples were analyzed by Low Pressure Vacuum Outlet Gas Chromatography coupled to a Triple Quadrupole Tandem Mass Spectrometry (LPGC-MS/MS).

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Filtration of Shellfish Extracts prior to final dilution for domoic acid (ASP; AMNESIC Shellfish Poisoning) analysis by HPLC

[A]mnesic shellfish poisoning (ASP) toxin, domoic acid, belongs to a group of amino acids, called the kainoids, which a...

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Screening and Quantitation of 250 Pesticides in Apple, Cranberry, Orange, Vegetable and White Grape Juices using the eXtreme|FV® by LC/MS/MS

[A]nalysis of 250 pesticides in store-bought juice using one method and simple sample preparation using the Thomson [eX...

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Routine Targeted Quantitation & Identification of Pesticide Residues in Avocado, Carrot, Grape & Orange using the eXtreme|FV® by LC-MS/MS

Thomson eXtreme|FV®s for clean-up, and the Sciex Scheduled MRM Pro Algorithm for identification of pesticides in fruit ...

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Tea Analysis with eXtreme|FV® by GC-MS

This method investigates whether SPE is required for the analysis of pesticides in green tea leaves using GC-MS.

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Screening and Quantitation of 200+ Pesticides in Honey by an Integrated On-Line Extraction UHPLC-MS/MS System

[A] simple, cost effective and sensitive procedure for screening and quantitation of pesticides in honey using the Thom...

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Analysis of Antibiotics in Honey by an Integrated On-Line Extraction UHPLC-MS/MS System

The most critical aspects of reliable food contamination analysis are the reduction of interferences from the sample ma...

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eXtreme|FV® vs SPE for the Analysis of Pesticides in Orange Juice by GC/MS

Pesticides act as toxins when found in sufficient quantities as residues in food. Solid Phase Extraction (SPE) is a com...

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Title

ASMS 2015 - Improved Sample Prep of Meat & Fish for Contaminate Analysis by LC/MS using Thomson eXtractor3D|FV™

Description

High-throughput analysis is needed to meet demand for monitoring of veterinary drug residues in food animal tissues. The current veterinary drug residue monitoring method used by the USDA Food Safety and Inspection Service (FSIS) uses a combination of hexane-partitioning, dispersive-SPE, and solvent evaporation to achieve adequate cleanup for 20 m...

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Product Used

eXtractor3D|FV® - PVDF 0.2µm

Non-Split Septum, Red Cap

pn#95531-200

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