Multiclass multiresidue analysis of >100 veterinary drug residues in bovine tissues by filter-vial dispersive-SPE and LC-MS/MS

Poster by USDA
Steven J. Lehotay, Marilyn J. Schneider, and Alan R. Lightfield
U. S. Department of Agriculture, Agricultural Research Service, Eastern Regional Research Center;
600 East Mermaid Lane; Wyndmoor, PA 19038; USA

High-throughput analysis is needed to meet demand for monitoring of veterinary drug residues in food animal tissues. The current veterinary drug residue monitoring method used by the USDA Food Safety and Inspection Service (FSIS) uses a combination of hexane-partitioning, dispersive-SPE, and solvent evaporation to achieve adequate cleanup for 20 mg equivalent sample injections to meet regulatory detection limit needs. This extra effort adds to the time and cost of the method and limits sample throughput. In this study, a new LC-MS/MS QTrap instrument was used which met the same detection limit needs with merely 0.17 mg equivalent sample injection. This obviated the hexane-partitioning and solvent evaporation steps, and filter-vial dispersive-SPE was used to eliminate a centrifugation step. In this way, sample turnaround time was 20 min for an individual sample, and sample throughput was doubled for a batch of 60 samples. More than 100 of the 130 tested drug analytes achieved 70-120% recoveries and <20% RSD for 90 replicate injections at 3 spiking levels over the course of 3 days in each of bovine kidney and muscle sample types. This method outperforms the current FSIS method and may be used in the future to provide quantitative as well as qualitative screening results in routine monitoring programs

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Title

Multiclass multiresidue analysis of >100 veterinary drug residues in bovine tissues by filter-vial dispersive-SPE and LC-MS/MS

Description

High-throughput analysis is needed to meet demand for monitoring of veterinary drug residues in food animal tissues. The current veterinary drug residue monitoring method used by the USDA Food Safety and Inspection Service (FSIS) uses a combination of hexane-partitioning, dispersive-SPE, and solvent evaporation to achieve adequate cleanup for 20 m...

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Product Used

eXtreme|FV® - PVDF 0.2µm

Pre-Split Septum, Red Cap

pn#85531-200

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