Thomson Well Plates

Thomson’s Well Plates represent a cross section of plates for various types of assays, cell growth and storage. We have the option in 96 and 24 well formats for scientists needs. Thomson Instrument Company additionally offers a variety of cap mats, lids, and seals for covering your culture plates.

Although we carry a catalog of plates in our inventory, here are our most popular for E. coli & microbial growth with our recommended protocol. To see our entire selection of well plates, see our Well Plates page


96 Well Screening Protocol for E. coli and other Microbes

Materials

  • 96 Well Plate, 2mL, Square Well, V-Bottom, Raised Lettering |Sterile: p/n 951652C
  • 96 Well Plate, 2mL, Square Well, Round Bottom | Irreversible: p/n 931130
  • Airporous Seal For Growing Cultures: p/n 899410

Methods

  1. Pipette 750uL of bacterial growth media containing the appropriate selective antibiotic into each well of a 96 well plate.
  2. Add the selected colony to each well from either an agar plate or glycerol stock.
  3. Gently triturate each well manually.
  4. Seal plates with the Airporous Seal and transfer to shaker overnight at 850rpm and 37°C.
  5. Harvest the plates by centrifugation @ 2500g for 20 minutes.
  6. Invert the plate to discard the media.
  7. Process samples according to downstream application (plasmid purification, protein extraction etc.)

Notes

  • 96 well cultures grown in Plasmid+ medium (p/n 446300) typically provide the appropriate biomass for MINI scale plasmid preps.
  • The most critical factor in cell viability is aeration. Optimal results will be achieved using shakers with 3mm orbit diameters. We do not recommend working in 96 well format using shakers with standard 25mm or 50mm throws.
  • If high levels of evaporation are encountered, the well plate & plastic lid (p/n 931134) is recommended to alleviate the issue.
  • Thomson Instrument Company’s filter plates are a great complimentary product for downstream purification applications. Add appropriate resin.
    • 96 Well Filter Plate, 2mL, Long Drip | 25µm Polypropylene: p/n 931919

Although we carry a catalog of plates in our inventory, here are our most popular for E. coli & microbial growth with 96 well plates in the above protocol. To see our entire selection of well plates, see our Well Plates page

Plate & Seal Part Numbers

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24 Well Growth Protocol for E. coli and Other Microbes

Materials

  • 24 Well Plate, 10.4mL, Square Well, Round Bottom, Individually Wrapped | Sterile: p/n 931565-G-1X
    • plus: Airporous Seal for Growing Cultures: p/n 899410
  • 24 Well Plate, 10.8mL, Square Well, Pyramid Bottom, Individually Wrapped | Sterile: p/n 931569-G-1X
    • plus: Airporous Seal for Growing Cultures: p/n 899410
  • 24 Well Plate, 10.4mL, Square Well, Round Bottom, Individually Wrapped with Lid | Sterile: p/n 931568
  • 24 Well Plate, 10.8mL, Square Well, Pyramid Bottom, Individually Wrapped with Lid | Sterile: p/n 931571

Methods

  1. Pipette 4-5mL of bacterial growth media containing the appropriate selective antibiotic into each well of a 24 well plate.
  2. Add the selected colony to each well from either an agar plate or glycerol stock.
  3. Gently triturate each well manually.
  4. Seal plates with airporous seals and transfer to shaker overnight at 350-400rpm and 37°C.
  5. Harvest the plates by centrifugation @ 2500g for 20 minutes.
  6. Invert the plate to discard the media.
  7. Process samples according to downstream application (plasmid purification, protein extraction etc.)

Notes

  • 24 well cultures grown in Plasmid+ medium (p/n 446300) typically provide the appropriate biomass for MIDI scale plasmid preps.
  • The most critical factor in cell viability is aeration. Optimal results will be achieved using shakers with 12.5mm (preferred), or 3mm orbit diameters. Cultures grown on shakers with standard 25mm throws will likely need increased rotational speed or decreased culture volume.
  • If high levels of evaporation are encountered, use a 24 well plate & plastic lid (p/n 931568) is recommended to alleviate the issue.

Although we carry a catalog of plates in our inventory, here are our most popular for E. coli & microbial growth with 24 well plates in the above protocol. To see our entire selection of well plates, see our Well Plates page

Plate & Seal Part Numbers

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