We have found many pumps in good working order will work well. The pump should be rated to be able to maintain 500rpm and accept Silicone Tubing, 3/16" ID x 3/8" OD x 4'.

Unlike other systems the Rapid Clear® System does not it require a manifold and constant vigilance over pressure readings. It is safe to set up, and walk away.

Silicone Tubing, 3/16" ID x 3/8" OD x 4'.

Optimal cell culture harvest conditions are approximately 70-90%. Cell Cultures under 70% viability contain proteases which chew up extra cellular proteins (component of interest), have high amounts of dead cell debris & heterogeneity in protein quality. Less than 70% viability requires additional purification steps which leads to more loss. Additional lose in purification is seen when stretching the day to get an extra 10% titer.

The Rapid Clear® Cap comes with an attached secondary flask cap that will fit the following Optimum Growth® Flasks: 1.6L, 2.8L or 5L.

The sterile Optimum Growth® Low Evaporation Cap, (p/n 899600-B), will fit the following Optimum Growth® Flasks: 1.6L, 2.8L & 5L.

The Rapid Clear® Cap will have foam, but after checking 1000 caps, we have not seen a degradation with foam, it is one of the "urban legends".

The Rapid Clear® Cap will only work with the Optimum Growth® Flasks.

At this time, The Rapid Clear® Cap will only work the 1.6L, 2.8L, & 5L Optimum Growth® Flasks. Rapid Clear® Caps for the 125mL, 250mL or 500mL Optimum Growth® Flasks will be coming soon.

Yes, the Rapid Clear® Cap is endotoxin free. The Rapid Clear® Cap will not remove endotoxin's that might be in the culture.

The Rapid Clear® filter does not need to be flushed prior to use. It comes out of the bag ready to go.

The Rapid Clear® filter is not designed for reverse flow. Do not run the pump in reverse.

Once the maximum cell capacity of the Rapid Clear® filter has been reached it will need to be replaced with a new Rapid Clear® filter. After replacing the filter ensure the flask contents have not settled prior to restarting the pump. Please note we recommend stopping the unit at 35mL/minute.

Do not allow the culture to sit on the bench for a long time prior to using the Rapid Clear® Cap. If needed, store culture in a refrigerator prior to use.

We have many customers who instead of letting cells go to low viability, put the flasks into the refrigerator at 70%+ viability, and then harvest in the morning.

It is best to keep air bubbles to a minimum during the filtration process. Air bubbles can be avoided by ensuring the dip tube end is submersed in the cell culture prior to starting the pump. Ratchet the clamp prior to starting and stopping the pump.

When the last few hundred milliliters remain in the Optimum Growth® Flask, placing a wedge under one side of the Optimum Growth® Flask to allow the dip tube to be immersed in the culture until it is empty; ratchet the clamp, stop the pump and add 400-500mL of PBS. This will ensure all the culture is filtered.