Thomson's Ultra Yield® system has proven over the last decade to enhance the aeration of E. coli and other microbial cells.
Thomson's Ultra Yield® System
The Ultra Yield® System consists of four components
Ultra Yield® Flasks
Ultra Yield® Flasks are designed to be either single-use or autoclaved up to 3x and feature a unique geometry that enhances gas exchange.
AirOtop® Enhanced Seals
AirOtop® Enhanced Seals and Vented Screw Caps feature a 0.2μm hydrophobic gas-permeable resealable barrier providing consistent gas exchange for the duration of your culture.
Plasmid+® Enriched media
Plasmid+® media is an animal origin free enhanced media which provides essential nutrients to help increase your DNA yields.
Ultra Yield® BiDirectional Transfer Cap
In addition, when it comes time to harvest flask contents or transfer to larger fermenters Thomson manufactures a single-use Ultra Yield® Bidirectional Transfer Cap for the 1.5L and 2.5L Ultra Yield® Flasks. This aseptic transfer system keeps your process sterile and GMP-compliant.
Ultra Yield® Flasks
Key Features
10x increased aeration over standard shake flasks
Increased DNA & protein production
Fully scalable results
Replacement for glass flasks
Fits all standard flask clamps
Easily adaptable into microbial growth protocols
Sterile, autoclavable flasks from 125mL - 2.5L
Use with AirOtop® Enhanced Seals or Vented Screw Caps and Plasmid+® media
Thomson is not affiliated with Kuhner or their products
Data from Pfizer: 610% Yield Increase
Economical parallel protein expression screening and scale-up in Escherichia coli. Journal of Structural and Functional Genomics2006 Jun;7(2):101-8. Epub 2006 Dec 23.
Effect of flask design on E.coli culture growth and production of recombinant protein. (A) The effect of flask type and growth medium on the observed optical densities of the cultures at A600 following overnight protein expression. Cultures carried out in Fernbach flasks using either LB medium or TB medium are shown, respectively at the front (green) and in the center (orange). Cultures grown in Ultra Yield® flasks in TB medium are shown at the back (blue). (B) The yields of expressed soluble protein (determined by protein assay and recorded as mg protein per liter of expression culture) from the IMAC columns following purification of the twelve polyHis-tagged recombinant proteins, each expressed under the three conditions described in Panel A. Clone locations in Panel B are the same as those in Panel A.
Thomson is not affiliated with Pfizer or its subsidiaries
Data from GSK
E. coli when cultured in Thomson Ultra Yield® Flasks show cells going from Log Phase to Transition Phase (as opposed to Stationary Phase) resulting in an increase in microbial OD, overall a more stable pH and a lower acetate level compared to glass flasks.
Thomson is not affiliated with Glaxo Smith Kline (GSK) or its subsidiaries
Visit More of the Ultra Yield® System
Recommended Shake Speeds
Recommended culture volumes and orbital shaker speeds (a throw of 1″ (2.54cm) is generally used for orbital shaking).
| Part # | Description | Use AirOtop® Seal / Vented Screw Cap | Media (mL)/Flask | Shaker Speed |
|---|---|---|---|---|
| 931147 | 125mL Ultra Yield® Flasks 50/case -- Sterile | 899421 / 899109 | 35-50mL/flask | 300-350 RPM |
| 931144 | 250mL Ultra Yield® Flasks 50/case -- Sterile | 899423 / 899110 | 75-100mL/flask | 300-350 RPM |
| 931141 | 500mL Ultra Yield® Flasks 25/case -- Sterile | 899424 / 899111 | 150-200mL/flask | 300-350 RPM |
| 931138 | 1.5L Ultra Yield® Flasks 12/case -- Sterile | 899425/899566 | 300mL/flask | 300-350 RPM |
| 931136-B | 2.5L Ultra Yield® Flask 6/case -- Sterile | 899425 / 899566 | 500mL/flask | 300-400 RPM |
Flask Clamp Compatibility
Refer to the chart below to find the appropriate flask clamp compatible with your shaker.
| Flask Size | Eppendorf® | INFORS HT® | Kuhner® | Fisher Scientific® | VWR® |
|---|---|---|---|---|---|
| 125mL | M1190-9001 | 12202 | SM310100 | 11-676-013 | 57019-676 |
| 250mL | M1190-9002 | 12203 | SM310250 | 11-676-014 | 57019-678 |
| 500mL | M1190-9003 | 12204 | SM310500 | 11-676-015 | 57019-682 |
| 1.5L | ACE1000S | 12205 | SM311000 | 11-676-016 | 57019-684 |
| 2.5L | M1190-9005 | 12206 | SM312500U | 11-676-017 | 57019-686 |
Nationwide Technical Support
We're here to help!
Thomson employs a nationwide team of expert technical support staff ready to tackle any questions you may have regarding our products.