Well Plates
Thomson Well Plates in both 6- 24- & 96-well configurations are ideal for suspension cell culturing and feature various well and well bottom shapes to suit your culture conditions
Choose one from each option to find the perfect match.
Thomson Well Plates
Standard depth and deep-well 6- 24- & 96-well configurations are ideal for suspension cell culturing and analytical sample preparation. To compliment Thomson well plates we also offer various sealing options including capmats, Airporous Seals, foil and plastic lid options
Key Features
Well Shape - Square or Round to fit your cell type and culture condition requirements
Well Bottom Shape - Pyramid, Round and V-bottom to fit your culturing, subculturing or DNA concentrating applications
Well Plate Orientation - Fixed for Robotic Liquid-Handling Systems
6-Well Plate Platform
A Mid-Scale Bioprocessing Solution
Designed for working volumes 20mL to 50mL
The Thomson 6-Well Plate with the integrative cap, is designed for mid-scale cell culture, supporting larger working volumes. The platform's integrated design ensures stable, contamination-free conditions that are essential for high quality bioprocessing applications, making it ideal for scaling CHO, HEK293, or insect cell workflows.
Key Features
Integrated Lid: Best use is 1-7-day growth
Large Working Volume Range: Supports 20mL to 50mL, ideal for flexible scale-up
High Throughput: Accommodates 24 plates per tray with universal shaker compatibility
User-Friendly: Eliminates spin tube handling issues like wrist strain from uncapping
Nationwide Technical Support
We're here to help!
Thomson employs a nationwide team of expert technical support staff ready to tackle any questions you may have regarding our products.
Cell Culture Protocols for 24- & 96-Well Plates
Thomson's well plates represent a cross section of plates for various types of assays, cell growth, and storage. We have options in 96 and 24-well formats for scientists' needs. Thomson additionally offers a variety of capmats, lids, and seals for covering your culture plates.
96-Well Screening Protocol for Mammalian/Insect Cells
Materials
- 96-Well Plate, 2mL, Square Well, Pyramid Bottom, Individually Wrapped with Lid | Sterile: p/n 931137
Methods
- Maintain cell stocks in appropriate growth medium. Split cultures the day before transfection to an appropriate density to ensure log phase growth at the time of transfection
- Seed cells at 500μL/well. The optimal seeding density will depend on the cell line, please use cell line recommended density
- Transfect cells according to established transfection protocol. Scale transfection reagent/DNA/feeds on a volumetric basis from what is used for larger scale cultures
- Seal plates with plastic lids or airporous seals and transfer to shaker overnight at 800rpm and 37°C
- Harvest cells at the time point established for larger scale cultures. Pellet cells by centrifugation at 2500g for 20min at 4°C
- Reserve either the culture media or the pellet depending on the application and proceed to downstream processing
Notes
- The most critical factor in cell viability is aeration. Optimal results will be achieved using shakers with 3mm orbit diameters. We do not recommend working in 96-well formation using shakers with standard 25mm throws
- Thomson filter plates are a great complimentary product for downstream purification applications
- 96-Well Filter Plate, 2mL, Long Drip | 25µm Polypropylene: p/n 931919
- Maximum suggested centrifugation: 3000g
24-Well Screening Protocol for Mammalian/Insect Cells
Materials
- 24-Well Plate, 10.4mL, Square Well, Round Bottom, Individually Wrapped with Lid | Sterile: p/n 931568
- 24-Well Plate, 10.8mL, Square Well, Pyramid Bottom, Individually Wrapped with Lid | Sterile: p/n 931571
Methods
- Maintain cell stocks in appropriate growth media. Split cultures the day before transfection to an appropriate density to ensure log phase growth at the time of transfection.
- Seed cells at 4-5mL/well. The optimal seeding density will depend on the cell line, please use cell line recommended density.
- Transfect cells according to established transfection protocol. Scale transfection reagent/DNA/feeds on a volumetric basis from what is used for larger scale cultures.
- Cover plates with plastic lids and transfer to shaker overnight at 350rpm and 37°C.
- Harvest cells at the time point established for larger scale cultures. Pellet cells by centrifugation at 2500g for 20min at 4°C.
- Reserve either the culture media or the pellet depending on the application and proceed to downstream processing.
Notes
- The most critical factor in cell viability is aeration. For 24-well plates optimal results will be achieved using shakers with 12.5mm. Cultures grown on shakers with standard 25mm throws, will likely need increased rotational speed or decreased culture volume.
96-Well Screening Protocol for E. coli and other Microbes
Materials
- 96-Well Plate, 2mL, Square Well, V-Bottom, Raised Lettering |Sterile: p/n 951652C
- 96-Well Plate, 2mL, Square Well, Round Bottom | Irreversible: p/n 931130
- Airporous Seal For Growing Cultures: p/n 899410
Methods
- Pipette 750uL of bacterial growth media containing the appropriate selective antibiotic into each well of a 96 well plate.
- Add the selected colony to each well from either an agar plate or glycerol stock.
- Gently triturate each well manually.
- Seal plates with the Airporous Seal and transfer to shaker overnight at 850rpm and 37°C.
- Harvest the plates by centrifugation @ 2500g for 20 minutes.
- Invert the plate to discard the media.
- Process samples according to downstream application (plasmid purification, protein extraction etc.)
Notes
- 96-well cultures grown in Plasmid+ medium (p/n 446300) typically provide the appropriate biomass for MINI scale plasmid preps.
- The most critical factor in cell viability is aeration. Optimal results will be achieved using shakers with 3mm orbit diameters. We do not recommend working in 96-well format using shakers with standard 25mm or 50mm throws.
- If high levels of evaporation are encountered, the well plate & plastic lid (p/n 931134) is recommended to alleviate the issue.
- Thomson Instrument Company's filter plates are a great complimentary product for downstream purification applications. Add appropriate resin.
- 96-Well Filter Plate, 2mL, Long Drip | 25µm Polypropylene: p/n 931919
24-Well Growth Protocol for E. coli and Other Microbes
Materials
- 24-Well Plate, 10.4mL, Square Well, Round Bottom, Individually Wrapped | Sterile: p/n 931565-G-1X
- plus: Airporous Seal for Growing Cultures: p/n 899410
- 24-Well Plate, 10.8mL, Square Well, Pyramid Bottom, Individually Wrapped | Sterile: p/n 931569-G-1X
- plus: Airporous Seal for Growing Cultures: p/n 899410
- 24-Well Plate, 10.4mL, Square Well, Round Bottom, Individually Wrapped with Lid | Sterile: p/n 931568
- 24-Well Plate, 10.8mL, Square Well, Pyramid Bottom, Individually Wrapped with Lid | Sterile: p/n 931571
Methods
- Pipette 4-5mL of bacterial growth media containing the appropriate selective antibiotic into each well of a 24 well plate.
- Add the selected colony to each well from either an agar plate or glycerol stock.
- Gently triturate each well manually.
- Seal plates with airporous seals and transfer to shaker overnight at 350-400rpm and 37°C.
- Harvest the plates by centrifugation @ 2500g for 20 minutes.
- Invert the plate to discard the media.
- Process samples according to downstream application (plasmid purification, protein extraction etc.)
Notes
- 24-well cultures grown in Plasmid+ medium (p/n 446300) typically provide the appropriate biomass for MIDI scale plasmid preps.
- The most critical factor in cell viability is aeration. For 24-well plates optimal results will be achieved using shakers with 12.5mm. Cultures grown on shakers with standard 25mm throws will likely need increased rotational speed or decreased culture volume.
- If high levels of evaporation are encountered, use a 24 well plate & plastic lid (p/n 931568) is recommended to alleviate the issue.
Looking For More Options With Well Plates, Covers & Lids?
Seals & Lids?
a variety of sealing options for all types of application
To complement our well plates Thomson also offers a variety of sealing options for all types of applications including:
- Gas-Permeable Airporous Seals for cell culture
- Aluminum Foil Adhesive and Heat Seals for short- and long-term storage
- Capmats for long-term storage
- Universal Plastic Lids
