Title
Optimization of Plasmid DNA Production in Escherichia coli Utilizing the Thermo Scientific MaxQ 8000 Incubated Stackable Shakers
Description
This application note describes an experiment used to optimize plasmid DNA yield in E. coli shake flask cultures using Thermo Scientific MaxQ 8000 incubated shakers. We were able to determine the effect that agitation speed and medium type had on cell density in shake flask cultures, and develop an optimized protocol for the production of...
Optimization of Plasmid DNA Production in Escherichia coli Utilizing the Thermo Scientific MaxQ 8000 Incubated Stackable Shakers
Meagan Gelinske, Microbiologist, Aldevron
Shyam Thapa, R&D Scientist, Aldevron
This application note describes an experiment used to optimize plasmid DNA yield in E. coli shake flask cultures using Thermo Scientific MaxQ 8000 incubated shakers. We were able to determine the effect that agitation speed and medium type had on cell density in shake flask cultures, and develop an optimized protocol for the production of plasmid DNA. This optimization resulted in increased plasmid DNA yields up to 16-fold higher than traditional growth protocols.