eXtreme|FV for sample prep prior to the analysis of cannabinoids by HPLC-UV

Introduction

Analysis of cannabinoids in marijuana flower, hemp and finished goods is becoming increasingly important as many states are legalizing it for medicinal and recreational purposes. Dosing methods include smoking/vaporizing and edibles but cannabis is still a Schedule 1 illegal drug and therefore have no FDA testing guidelines. This study evaluates streamlining the sample preparation aspect for HPLC-UV analysis of a panel of cannbinoids.

The following analytes were used

  • Cannabinol (CBN)
  • Cannabidivarinic acid (CBDVA)
  • Cannabinolic acid (CBNA)
  • Cannabidivarin (CBDV)
  • Δ9-Tetrahydrocannabinol (Δ9-THC)
  • Cannabidiolic acid (CBDA)
  • Δ8-Tetrahydrocannabinol (Δ8-THC)
  • Cannabigerolic acid (CBGA)
  • Cannabicyclol (CBL)
  • Cannabigerol (CBG)
  • Cannabichromene (CBC)
  • Cannabidiol (CBD)
  • Tetrahydrocannabinolic acid A (THCA-A)
  • Tetrahydrocannabivarin (THCV)
  • Cannabichromenic acid (CBCA)
  • Tetrahydrocannabivarinic acid (THCVA)

Equipment

HPLC: Shimadzu Prominance
UV/VIS:228nm

Column:Raptor ARC, 150 mm x 4.6 mm ID
Column Temperature:30 °C
Flow Rate:1.5mL/min

Mobile Phase:
A: 25%: Water, 5 mM ammonium formate, 0.1% Formic Acid
B: 75%: Acetonitrile, 0.1% Formic Acid

Sample Preparation

  • Place 200uL of sample into the outer shell of the eXtreme|FV, 0.2um PVDF
  • Add 200uL of 25:75 (Water:Methanol)
  • Partially depress the plunger
  • Vortex the sample
  • Depress the plunger completely

Results

16 cannabinoids are baseline resolved using the eXtreme|FV for sample prep and a isocratic HPLC method.

Peaks #AnalyteTime (min)
1Cannabidivarinic acid (CBDVA)1.877
2Cannabidivarin (CBDV)2.86
3Cannabidiolic acid (CBDA)2.592
4Cannabigerolic acid (CBGA)2.75
5Cannabigerol (CBG)2.912
6Cannabidiol (CBD)3.48
7Tetrahydrocannabivarin (THCV)3.391
8Tetrahydrocannabivarinic acid (THCVA)4.279
9Cannabinol (CBN)4.609
10Cannabinolic acid (CBNA)5.437
11Δ9-Tetrahydrocannabinol (Δ9-THC)5.815
12Δ8-Tetrahydrocannabinol (Δ8-THC)6.2
13Cannabicyclol (CBL)6.916
14Cannabichromene (CBC)7.263
15Tetrahydrocannabinolic acid A (THCA-A)7.612
16Cannabichromenic acid (CBCA)8.51
APP fig analysis of cannabinoids 001

Conclusion

The HPLC method fully resolves 16 major and minor cannabinoids. Simple quick sample prep using the eXtreme|FV allows for the baseline separation of the analytes ensuring positive identification and accurate quantitation of the cannabinoids. With <10 seconds per sample and a fast 9-minute analysis, all compounds were resolved making this method suitable for high-throughput cannabis testing labs.

Title

eXtreme|FV® for sample prep prior to the analysis of cannabinoids by HPLC-UV

Description

Analysis of cannabinoids in marijuana flower, hemp and finished goods is becoming increasingly important as many states are legalizing it for medicinal and recreational purposes. Dosing methods include smoking/vaporizing and edibles but cannabis is still a Schedule 1 illegal drug and therefore have no FDA testing guidelines. This study evaluates s...

PDF Application

Product Used in Application

eXtreme|FV® - PVDF 0.2µm

Pre-Split Septum, Red Cap

pn#85531-200

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