Title
Optimizing Plasmid Yields - Impact of Flask Design, Media Type and Shaking Parameters
Description
We examine the influence of culture media, vessel design, fill volume and shaking speed on bacteria and pDNA yields. We illustrate how larger production range can be obtained by using the appropriate combination between a large incubated shaker, optimized culture conditions and specifically-designed culture flasks.
Products Used in Application
Ultra Yield® 2.5L Flask
500mL-1L Working Volume | Sterile
pn#931136-B
Plasmid+® Media
1L Bottle | Sterile
pn#446300
Related Products
Optimizing Plasmid Yields - Impact of Flask Design, Media Type and Shaking Parameters
Blandine Vanbellinghen1, Silvia Tejerina1, Aurelie Tacheny1, Ines Hartmann2
1Eppendorf Application Technologies, S.A., Namur, Belgium
2Eppendorf SE, Hamburg, Germany
1Eppendorf Application Technologies, S.A., Namur, Belgium
2Eppendorf SE, Hamburg, Germany
We examine the influence of culture media, vessel design, fill volume and shaking speed on bacteria and pDNA yields. We illustrate how larger production range can be obtained by using the appropriate combination between a large incubated shaker, optimized culture conditions and specifically-designed culture flasks.
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