Analysis of Nitrosamines in Tobacco with eXtreme|FV® by LC-MS

Introduction

Tobacco-specific nitrosamines (TSNA) are a group of carcinogens found only in tobacco products. They are formed from nicotine and related alkaloids during the production and processing of tobacco and tobacco products. In recent years due to their carcinogenic properties, efforts have been made to reduce TSNA levels in tobacco products. The desired goal of this investigation is to develop a sensitive, high-throughput method to monitor TSNA levels in tobacco and tobacco products. This method descripes a simple robust sample preparation utilizing the Thomson Filter Vials for in-vial filtration: N’-nitrosonornicotine (NNN), N’-nitrosoanatabine (NAT), N-nitrosoanabasine (NAB), 4-(Methylnitrosamino)-1-(3-pyridyl)-1-butanol (NNAL) and 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK).

Prep

  1. 0.25g of unburned/smokeless tobacco sample
  2. Extracted with 100mM ammonium acetate solution, filtered with eXtreme|FV® PVDF 0.45 µm

Equipment

HPLC

Injection Volume:5µL
Column:Waters Xterra MS C18, 50x4.6mm, 5µm
Aqueous phase:5mM ammonium acetate in HPLC water
Organic Phase:5mM ammonium acetate in 95/5 acetonitrile/water

Gradient

Time [min]Organic %
05
15
235
535
65
85

  • Flow rate: 1mL/min
  • Temperature: 60°C
  • Detection: MS/MS
AnalyteIon pair Q1/Q3 (amu)
N-Nitrosoanabasine (NAB)192/162
N-Nitrosoanatabine (NAT)190/160
N-Nitrosonornicotine (NNK)208/122
4-(Methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNN)178/148
4-(Methylnitrosamino)-1-(3-pyridyl)-1-butanol (NNAL)210/180
tobacco

Title

Analysis of Nitrosamines in Tobacco with eXtreme|FV® by LC-MS

Description

[N]itrosamines (TSNA) are a group of carcinogens found only in tobacco ... method describes a simple robust sample preparation utilizing the Thomson Filter Vials for in-vial filtration.

PDF Application

Product Used in Application

eXtreme|FV® - PVDF 0.45µm

Pre-Split Septum, Yellow Cap

pn#85541-200

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