Pesticide analysis of cannabis leaves and finished goods is becoming increasingly important as many states are legalizing it for medicinal and recreational purposes. Dosing methods include smoking/vaporizing and edibles but cannabis is still a Schedule 1 illegal drug and therefore have no FDA testing guidelines. Trace levels of pesticides can be incurred during cultivation or inhaled from dried pesticides on the cannabis. This study evaluates the sample preparation aspect for LC-MS/ MS analysis of a 50+ analyte panel of pesticides, fungicides and aflatoxins. QuEChERS was used to extract the analytes from the cannabis flowers, followed by centrifugation and Thomson Standard Filter Vial for sample clean-up.