Filtration of Shellfish Extracts prior to final dilution for domoic acid (ASP; AMNESIC Shellfish Poisoning) analysis by HPLC

Data supplied by Canadian Food Inspection Agency

Introduction

The amnesic shellfish poisoning (ASP) toxin, domoic acid, belongs to a group of amino acids, called the kainoids, which are classed as neuroexcitants or excitoxins that interfere with the neurotransmission mechanisms in the brain. The toxin can be accumulated in shellfish feeding on a number of toxic Pseudonitzschia species. Ingestion of seafood contaminated with domoic acid can lead to an intoxication which symptoms include (among others) abdominal cramps, vomiting, disorientation and memory loss (amnesia) and can become severe in certain cases. The Thomson Filter Vials were evaluated for inclusion in the validated method, replacing filtration using a syringe, syringe filter and transfer to the HPLC Autosampler Vial.

shellfish 1 02

fig.1Clams spiked with Domoic Acid (5 µg/g); extract filtered using typical Nylon Syringe Filter

shellfish 2 03

fig.2Clams spiked with Domoic Acid (5 µg/g); extract filtered using 0.45 µm PVDF Filter Vial

Figure 1 shows typical injection chromatogram of clams extract seeded in domoic acid and filtrated using common filtration with Nylon Syringe Filter de 0.45µm.

Figure 2 shows same extract but filtrated with PVDF eXtreme Filter Vial 0.45µm from Thomson.

Both chromatograms and quantitative results are similars so filter vials filtration represent a faster suitable alternative for domoic acid in clams filtration method minimizing mistakes due to sample transfer.

Thomson Instrument Company is not affiliated with The Canadian Food Inspection Agency

Title

Filtration of Shellfish Extracts prior to final dilution for domoic acid (ASP; AMNESIC Shellfish Poisoning) analysis by HPLC

Description

[A]mnesic shellfish poisoning (ASP) toxin, domoic acid, belongs to a group of amino acids, called the kainoids, which are classed as neuroexcitants or excitoxins that interfere with the neurotransmission mechanisms in the brain. ... Filter Vials were evaluated for inclusion in the validated method, replacing filtration using a syringe, syringe fil...

PDF Application

Product Used in Application

eXtreme|FV® - PVDF 0.45µm

Pre-Split Septum, Yellow Cap

pn#85541-200

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