Title
Improved Sample Prep of Fruit & Juices for Analysis by Mass Spectrometry Using Thomson Filter Vials
Description
LC-MS/MS is a powerful analytical tool capable of screening samples for numerous compounds. MRM is typically used because of its excellent sensitivity, selectivity, and speed. Using QuEChERS for extraction, eXtreme|FV®s for clean-up, and UHPLC combined with coreshell particles columns provides good resolution and excellent peak shape, making it po...
Improved Sample Prep of Fruit & Juices for Analysis by Mass Spectrometry Using Thomson Filter Vials
Multiple papers used:
Routine Targeted Quantitation & Identification of Pesticide Residues in Spinach & Carrot using the eXtreme|FV® by LC-MS/MS
Screening and Quantitation of 250 Pesticides in Apple & Grape Juice using the eXtreme|FV® by LC/MS/MS
Comparison of eXtreme Filter Vials vs SPE for Improved Pesticide Recovery in Orange Juice by GC/MS
LC-MS/MS is a powerful analytical tool capable of screening samples for numerous compounds. MRM is typically used because of its excellent sensitivity, selectivity, and speed. Using QuEChERS for extraction, eXtreme|FV®s for clean-up, and UHPLC combined with coreshell particles columns provides good resolution and excellent peak shape, making it possible to detect hundreds of pesticides of a wide variety of compound classes and chemical properties in each sample. The new AB SCIEX Triple Quad™ 3500 with a Turbo V™ source and Curtain Gas™ interface supplies exceptional robustness and ruggedness. The advanced eQ™ electronics and the curved LINAC® collision cell were designed for unparalleled speed of MRM detection and fast polarity switching for comprehensive multi-component analysis. The method combines QuEChERS for extraction, Thomson eXtreme|FV®s for clean-up, and the Sciex Scheduled MRM Pro Algorithm for identification of pesticides in fruit and vegetables analysis.
A study was conducted using the Bruker EVOQ for the analysis of 250 pesticides in apple juice using only one method in store-bought juice and simple sample preparation using the Thomson eXtreme|FV®s in a dilute-and-shoot approach without sample enrichment. LCMS/MS operated in Multiple Reaction Monitoring (MRM) mode with dual scan Electrospray Ionization (ESI) is widely used for polar, semivolatile, and thermally labile pesticides in food testing. The Bruker EVOQ Elite LC-Triple Quadrupole System provides fast positive/negative switching, allowing for simultaneous determination of positive and negative co-eluting compounds numbering in the hundreds. Simple sample preparation is explored using Thomson eXtreme|FV®s for sample clean-up instead of lengthy alternatives like SPE or centrifugation followed by liquid-liquid extraction.
Pesticides are toxic when found in sufficient quantities as pesticide residue in food. This is of particular importance for orange juice because it is consum...