Evaluating the Impact of High Pluronic® F68 Concentrations on Antibody Producing CHO Cell Lines

Tharmala Tharmalingam, Chetan T. Goudar
Cell Science & Technology, Process & Product Development, Amgen Inc. One Amgen
Center Drive, Thousand Oaks 91320, California; telephone: 8054475321;
fax: 805-499-6819; e-mail: cgoudar@amgen.com

Routine analysis of samples containing cell culture media and/or supernatant by HPLC can lead to performance issues and can require frequent purchasing of new columns and increased HPLC maintenance. ... Here, we demonstrate that using Thomson [filter vials]] during sample preparation results in minimal impact preparation time, no detectable loss in product, and improved column performance leading to extended column lifetimes.

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Title

Evaluating the Impact of High Pluronic® F68 Concentrations on Antibody Producing CHO Cell Lines

Description

Routine analysis of samples containing cell culture media and/or supernatant by HPLC can lead to performance issues and can require frequent purchasing of new columns and increased HPLC maintenance. ... Here, we demonstrate that using Thomson [filter vials]] during sample preparation results in minimal impact preparation time, no detectable loss i...

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Product Used

Optimum Growth™ 125mL Flask

PTFE 0.2µm Vent Cap for Increased Aeration | Sterile

pn#931110

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