Economical parallel protein expression screening and scale-up in Escherichia coli

Oleg Brodsky, Ciarán N. Cronin

A novel microfermentation and scale-up platform for parallel protein production in Escherichia colii> is described. The vertical shaker device Vertiga, which generates low-volume high density (A600 ~ 20) Escherichia coli cultures in 96-position deep-well plates without auxiliary oxygen supplementation, has been coupled to a new disposable shake flask design, the Ultra Yield™ flask, that allows for equally high cell culture densities to be obtained. The Ultra Yield™ flask, which accommodates up to 1L in culture volume, has a baffled base and a more vertical wall construction compared to traditional shake flask designs. Experimental data is presented demonstrating that the Ultra Yield™ flask generates, on average, an equivalent amount of recombinant protein per unit cell culture density as do traditional shake flask designs but at a substantially greater amount per unit volume. The combination of Vertiga and the Ultra Yield™ flask provides a convenient and scalable low-cost solution to parallel protein production in Escherichia coli.

Related Application Notes

Improved DNA Protocol for E.coli with Plasmid+® Media

Plasmid+® liquid media is an enriched media specifically designed for plasmid DNA production. Plasmid+® supports much h...

See Application Note

Title

Economical parallel protein expression screening and scale-up in Escherichia coli

Description

A novel microfermentation and scale-up platform for parallel protein production in Escherichia colii> is described. The vertical shaker device Vertiga, which generates low-volume high density (A600 ~ 20) Escherichia coli cultures in 96-position deep-well plates without auxiliary oxygen supplementation, has been coupled to a new disposable s...

PDF Download

Product Used

Ultra Yield® 2.5L Flask

500mL-1L Working Volume | Sterile

pn#931136-B

Related Products

© 2021 Thomson |
Solutions At Work™

LinkedIn