Title
Crystal Structure of a Novel Dimeric Form of NS5A Domain I Protein from Hepatitis C Virus
Description
A new protein expression vector design utilizing an N-terminal six-histidine tag and tobacco etch virus protease cleavage site upstream of the hepatitis C virus NS5A sequence has resulted in a more straightforward purification method and improved yields of purified NS5A domain I protein.
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Crystal Structure of a Novel Dimeric Form of NS5A Domain I Protein from Hepatitis C Virus
Robert A. Love*, Oleg Brodsky, Michael J. Hickey, Peter A. Wells and Ciarán N. Cronin Structural Biology Group, Pfizer Global Research and Development, La Jolla Laboratories, San Diego, California 92121 *Corresponding author
A new protein expression vector design utilizing an N-terminal six-histidine tag and tobacco etch virus protease cleavage site upstream of the hepatitis C virus NS5A sequence has resulted in a more straightforward purification method and improved yields of purified NS5A domain I protein.
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