Title
High-yield production of biologically active recombinant protein in shake flask culture by combination of enzyme-based glucose delivery and increased oxygen transfer
Description
[R]eport describes the combined use of an enzyme-based glucose release system (EnBase®) and high-aeration shake flask (Ultra Yield Flask™). The benefit of this combination is demonstrated by over 100-fold improvement in the active yield of recombinant alcohol dehydrogenase expressed in E. coli
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High-yield production of biologically active recombinant protein in shake flask culture by combination of enzyme-based glucose delivery and increased oxygen transfer
Kaisa Ukkonen1,2*, Antti Vasala1, Heikki Ojamo2 and Peter Neubauer3
1BioSilta Oy, P. O. Box 4300, FI-90014 Oulu, Finland. 2Bioprocess Engineering Laboratory, Department of Process and Environmental Engineering, University of Oulu, P. O. Box 4300, FI-90014 Oulu, Finland. 3Chair of Bioprocess Engineering, Department of Biotechnology, Technische Universität Berlin, Ackerstrasse 71-76, D-13355 Berlin, Germany.
1BioSilta Oy, P. O. Box 4300, FI-90014 Oulu, Finland. 2Bioprocess Engineering Laboratory, Department of Process and Environmental Engineering, University of Oulu, P. O. Box 4300, FI-90014 Oulu, Finland. 3Chair of Bioprocess Engineering, Department of Biotechnology, Technische Universität Berlin, Ackerstrasse 71-76, D-13355 Berlin, Germany.
[R]eport describes the combined use of an enzyme-based glucose release system (EnBase®) and high-aeration shake flask (Ultra Yield Flask™). The benefit of this combination is demonstrated by over 100-fold improvement in the active yield of recombinant alcohol dehydrogenase expressed in E. coli
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