High-yield production of biologically active recombinant protein in shake flask culture by combination of enzyme-based glucose delivery and increased oxygen transfer

Kaisa Ukkonen<sup>1,2*</sup>, Antti Vasala<sup>1</sup>, Heikki Ojamo<sup>2</sup> and Peter Neubauer<sup>3</sup><br/> <sup>1</sup>BioSilta Oy, P. O. Box 4300, FI-90014 Oulu, Finland. <sup>2</sup>Bioprocess Engineering Laboratory, Department of Process and Environmental Engineering, University of Oulu, P. O. Box 4300, FI-90014 Oulu, Finland. <sup>3</sup>Chair of Bioprocess Engineering, Department of Biotechnology, Technische Universität Berlin, Ackerstrasse 71-76, D-13355 Berlin, Germany.

[R]eport describes the combined use of an enzyme-based glucose release system (EnBase®) and high-aeration shake flask (Ultra Yield Flask™). The benefit of this combination is demonstrated by over 100-fold improvement in the active yield of recombinant alcohol dehydrogenase expressed in E. coli

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Title

High-yield production of biologically active recombinant protein in shake flask culture by combination of enzyme-based glucose delivery and increased oxygen transfer

Description

[R]eport describes the combined use of an enzyme-based glucose release system (EnBase®) and high-aeration shake flask (Ultra Yield Flask™). The benefit of this combination is demonstrated by over 100-fold improvement in the active yield of recombinant alcohol dehydrogenase expressed in E. coli

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Product Used

Ultra Yield® 2.5L Flask

500mL-1L Working Volume | Sterile

pn#931136-B

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