Title
A Platform to Rapidly Purify and Formulate Immunoglobulins from Mammalian Expression Systems
Description
At Aragen, our typical steps to purify and formulate human 190 from transient system cultures up to 2 L involve clarification of harvest, affinity purification using Protein A and a combination of dialysis and concentration using centrifugal filter units for formulation. This platform can take from 5 to 7 days to complete, including assessment of ...
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PTFE 0.2µm Vent Cap for Increased Aeration | Sterile
pn#931116
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A Platform to Rapidly Purify and Formulate Immunoglobulins from Mammalian Expression Systems
Rene Pagila, Indira Kottayil, Nikita Patel, Sharleen Rojas, Sonam Sharma, Felix Vega, Jacqueline Wilde, Verginie Manard-Rose, Timothy Myles
At Aragen, our typical steps to purify and formulate human 190 from transient system cultures up to 2 L involve clarification of harvest, affinity purification using Protein A and a combination of dialysis and concentration using centrifugal filter units for formulation. This platform can take from 5 to 7 days to complete, including assessment of the formulated antibody for purity. Recently introduced technologies such as Protein A membrane devices and TFF systems capable of handling ultrafiltration and diafiltration of samples as low as 1 mL have tremendously decreased the turnaround time down to 3 days. The typical and improved platforms will be compared.