Title
A Platform to Rapidly Purify and Formulate Immunoglobulins from Mammalian Expression Systems
Description
At Aragen, our typical steps to purify and formulate human 190 from transient system cultures up to 2 L involve clarification of harvest, affinity purification using Protein A and a combination of dialysis and concentration using centrifugal filter units for formulation. This platform can take from 5 to 7 days to complete, including assessment of ...
Products Used

Optimum Growth® 5L Flask
PTFE 0.2µm Vent Cap for Increased Aeration | Sterile
pn#931116

Double Bagged Optimum Growth® 5L Flask
PTFE 0.2µm Vent Cap for Increased Aeration | Sterile
pn#931116-DB

Rapid Clear® Cap 3000
High-speed clarification of 2L–4L of cell culture | Sterile
pn#788116
Related Products

Optimum Growth® 125mL Flask
PTFE 0.2µm Vent Cap for Increased Aeration | Sterile
pn#931110

Double Bagged Optimum Growth® 125mL Flask
PTFE 0.2µm Vent Cap for Increased Aeration | Sterile
pn#931110-DB

Multiported Optimum Growth® 125mL Flask
PTFE 0.2µm Vent Cap for Increased Aeration | Sterile
pn#931110-DP

125mL 8 Position Carrier
Use with Optimum Growth® 125mL Flask (931110)
pn#1212900
A Platform to Rapidly Purify and Formulate Immunoglobulins from Mammalian Expression Systems
Rene Pagila, Indira Kottayil, Nikita Patel, Sharleen Rojas, Sonam Sharma, Felix Vega, Jacqueline Wilde, Verginie Manard-Rose, Timothy Myles
At Aragen, our typical steps to purify and formulate human 190 from transient system cultures up to 2 L involve clarification of harvest, affinity purification using Protein A and a combination of dialysis and concentration using centrifugal filter units for formulation. This platform can take from 5 to 7 days to complete, including assessment of the formulated antibody for purity. Recently introduced technologies such as Protein A membrane devices and TFF systems capable of handling ultrafiltration and diafiltration of samples as low as 1 mL have tremendously decreased the turnaround time down to 3 days. The typical and improved platforms will be compared.
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See Application NoteComparison of the Rapid Clear® Cap 3000 (patented) vs GE Capsule Filters for clarifying monospecific IgG
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See Application Note