A Platform to Rapidly Purify and Formulate Immunoglobulins from Mammalian Expression Systems

Poster Presented at PEGS 2017
Rene Pagila, Indira Kottayil, Nikita Patel, Sharleen Rojas, Sonam Sharma, Felix Vega, Jacqueline Wilde, Verginie Manard-Rose, Timothy Myles

At Aragen, our typical steps to purify and formulate human 190 from transient system cultures up to 2 L involve clarification of harvest, affinity purification using Protein A and a combination of dialysis and concentration using centrifugal filter units for formulation. This platform can take from 5 to 7 days to complete, including assessment of the formulated antibody for purity. Recently introduced technologies such as Protein A membrane devices and TFF systems capable of handling ultrafiltration and diafiltration of samples as low as 1 mL have tremendously decreased the turnaround time down to 3 days. The typical and improved platforms will be compared.

Related Application Notes

Comparison of the Rapid Clear® Cap 3000 (patented) vs GE Capsule Filters for clarifying monospecific IgG

Two methods to clarify 10L of post transfected IgG harvested from culture are compared. The culture was harvested on da...

See Application Note

Optimum Growth™ Protocol for Insect Cells

... Sf9 and Sf21 insect cells are cell lines commonly used for expression of recombinant proteins using baculovirus sys...

See Application Note

Simplified Sample Prep for Open Access SEC-HPLC Detection using the Thomson Filter Vials

Routine analysis of samples containing cell culture media and/or supernatant by HPLC can lead to performance issues and...

See Application Note

Improved DNA Protocol for E.coli with Plasmid+® Media

Plasmid+® liquid media is an enriched media specifically designed for plasmid DNA production. Plasmid+® supports much h...

See Application Note

Title

A Platform to Rapidly Purify and Formulate Immunoglobulins from Mammalian Expression Systems

Description

At Aragen, our typical steps to purify and formulate human 190 from transient system cultures up to 2 L involve clarification of harvest, affinity purification using Protein A and a combination of dialysis and concentration using centrifugal filter units for formulation. This platform can take from 5 to 7 days to complete, including assessment of ...

PDF Download

Products Used

Rapid Clear® Cap 3000

High-speed clarification of 2L–4L of cell culture | Sterile

pn#788116

Optimum Growth™ 5L Flask

PTFE 0.2µm Vent Cap for Increased Aeration | Sterile

pn#931116

Related Products

© 2021 Thomson |
Solutions At Work™

LinkedIn