New Innovation: Harvesting CHO and HEK293 Cells with the Rapid Clear® Cap provides 0.2μm filtration in a fraction of the time and requires less consumables than other clarification methods

Author: Daniel Korostyshevsky, Sam Ellis
Thomson Instrument Company, Oceanside, CA

When producing biologics, cell yield, viability, and effective clarification are critical. Thomson’s patented Optimum Growth™ Flask facilitates scalable mixing and high gas exchange rates to produce high density yields of viable cells. Now, to extend the Optimum Growth™ Flask product line into downstream processing, Thomson has developed a revolutionary new technology to perform high speed clarification of cellular material. The Rapid Clear Cap® 3000 eliminates the multiple transfer steps involved in clarification and will filter between 2L-4L of high density culture in under 35 minutes. This technique transforms the time consuming and laborious process of harvesting cells to a rapid, eco-friendly walk away procedure.

Related Application Notes

Comparison of the Rapid Clear® Cap 3000 (patented) vs GE Capsule Filters for clarifying monospecific IgG

Two methods to clarify 10L of post transfected IgG harvested from culture are compared. The culture was harvested on da...

See Application Note

Optimum Growth™ Protocol for Insect Cells

... Sf9 and Sf21 insect cells are cell lines commonly used for expression of recombinant proteins using baculovirus sys...

See Application Note

Simplified Sample Prep for Open Access SEC-HPLC Detection using the Thomson Filter Vials

Routine analysis of samples containing cell culture media and/or supernatant by HPLC can lead to performance issues and...

See Application Note

Improved DNA Protocol for E.coli with Plasmid+® Media

Plasmid+® liquid media is an enriched media specifically designed for plasmid DNA production. Plasmid+® supports much h...

See Application Note

Title

New Innovation: Harvesting CHO and HEK293 Cells with the Rapid Clear® Cap provides 0.2μm filtration in a fraction of the time and requires less consumables than other clarification methods

Description

When producing biologics, cell yield, viability, and effective clarification are critical. Thomson’s patented Optimum Growth™ Flask facilitates scalable mixing and high gas exchange rates to produce high density yields of viable cells. Now, to extend the Optimum Growth™ Flask product line into downstream processing, Thomson has developed a revolut...

PDF Download

Products Used

Rapid Clear® Cap 3000

High-speed clarification of 2L–4L of cell culture | Sterile

pn#788116

Optimum Growth™ 5L Flask

PTFE 0.2µm Vent Cap for Increased Aeration | Sterile

pn#931116

Related Products

© 2021 Thomson |
Solutions At Work™

LinkedIn