Title
Strategies to maximize heterologous protein expression in Escherichia coli with minimal cost
Description
Automation and miniaturization are key issues of high-throughput research projects in the post-genomic era. ... In this review, we describe recent efforts to continue to minimize the cost for the parallel processing of multiple protein targets and focus on those materials and strategies that are highly suitable for the traditional academic laborat...
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Strategies to maximize heterologous protein expression in Escherichia coli with minimal cost
Wolfgang Peti1, Rebecca Page2
1 Brown University, Department of Molecular Pharmacology, Physiology, and Biotechnology, Box G-E3, Providence, RI 02912, USA
2 Brown University, Department of Molecular Biology, Cell Biology and Biochemistry, Box G-E4, Providence, RI 02912, USA
1 Brown University, Department of Molecular Pharmacology, Physiology, and Biotechnology, Box G-E3, Providence, RI 02912, USA
2 Brown University, Department of Molecular Biology, Cell Biology and Biochemistry, Box G-E4, Providence, RI 02912, USA
Automation and miniaturization are key issues of high-throughput research projects in the post-genomic era. ... In this review, we describe recent efforts to continue to minimize the cost for the parallel processing of multiple protein targets and focus on those materials and strategies that are highly suitable for the traditional academic laboratory.