Standard Filter Vial Specifications

What is a Filter Vial?

Thomson Filter Vials (patented) are a single system which replaces HPLC Vials, HPLC Caps, Syringes, & Syringe Filters for the filtration of samples. In 15 seconds, Thomson Filter Vials allow for sample preparation of unfiltered samples to filtered samples in an autosampler-ready vial.

The Filter Vial consists of two parts: an outer shell shell and a plunger which includes a filter on one end and a vial cap on the other end. Samples are filtered by pipetting the sample into the filter vial outer shell, inserting the plunger, and pushing the plunger into the shell.

Filter Vials are the same size as HPLC vial

Same Size as HPLC Vial

Thomson Filter Vials are the same size as standard HPLC vials and will fit easily into any machine or tray available for standard HPLC vials.

Filter Vials are like HPLC vials with a syringe filter built in

Syringe Filter Built In

Filter Vials are equivalent to a syringe filter built into your HPLC vial. Even a sample that appear clear to the eye potentially have particulates that can clog the machine and cause down time and costly maintenance. Filter Vials increase productivity by eliminating a transfer step required when using a syringe filter.


Easy & Fast to Use

  1. Deposit 450µL of sample into shell vial
  2. Insert plunger into the outer shell & press

In two steps and 15 seconds you can have a safe and secure sample for analysis. If you need to filter more than one sample the use of a Toggle Press (up to 5) or Multi-Use Press (up to 48) can be used.

You can prepare a safe particulate free sample in less time than it takes to in the time it takes to open the syringe packaging and add the syringe filter.

Diagram showing the Thomson Filter Vial in action

How Filter Vials Work

Similar to how a french press (cafetière à piston) works, Filter Vials filter particulates out of the sample with similar membranes used in syringe filters. The pressing of the plunger into the shell vial forces the sample up through a filter to separate the particulates from the sample to be analyzed. Thomson has several filter membranes and pore sizes to choose from making the Filter Vial a versatile tool in the lab.


Membrane Pore Size

The recommended membrane pore size for sample filtration is based on the cell or cell debris content of the sample and the particle size of the packing material in the chromatography column used to analyze the sample. If the sample contains cells or cellular debris, then a 0.2μm pore size membrane is recommended to maintain system sterility.

Which to use?

  • 0.2μm Pore Size
    • Cells or Cell Debris in Sample
    • Chromatography Column Particle Size <3µm
  • 0.45μm Pore Size
    • Chromatography Column Particle Size >3µm

Membrane Material

The recommended membrane for sample filtration is based on the percentage of organic solvent in the sample and the amount of protein binding.

For chemical or compound compatibility with our Filter Vials & membranes see the Chemical Compatibility Index & Compound Compatibility Index in our Technical Library

Aqueous>50% OrganicLow Protein Binding
PTFE page/fv-bottle-icon.png
PVDF page/fv-drop-icon.png page/fv-dna-icon.png
Nylon page/fv-drop-icon.png page/fv-bottle-icon.png
PES page/fv-drop-icon.png page/fv-dna-icon.png

Types of Filter Vials

page/fv-logo-std.png

120μL Dead Volume

Max Fill Vol. 450µl
Dead Vol. 120µl

Thomson Standard Filter Vials can be used for samples containing less than 10% solid particulates. The filter vial consists of two parts: a filter vial shell and a plunger which includes a single layer filter on one end and a vial cap on the other end. Applications for Standard Filter Vials include filtration of catalysts from organic and medicinal chemistry synthesis reactions, saccharide analysis in corn syrup, and in-vial protein precipitation.

page/fv-logo-nano.png

10µL Minimum Volume

Max Fill Vol. 250µl
Min Fill Vol. 10µl (for 2µl injection)

Thomson nano|Filter Vials® offer a very low dead volume, allowing one to filter as little as 10µL of sample with enough remaining filtrate to make a 2µL injection.

Applications include all low volume samples: analysis of enzymes, peptides, DNA, RNA, synthesis reaction intermediates, finished products, saliva.

page/fv-logo-ext.png

Multi-Layered Filtration

Max Fill Vol. 450µl
Dead Vol. 120µl

Thomson eXtreme|FV® offer multi-layer filtration for viscous samples and samples containing up to 30% solid particulates.

eXtreme|FV® allows for compounds to be separated from the matrix, which results in both a higher signal-to-noise ratio and peaks that are more differentiated.

Prior to the introduction of the eXtreme|FV®, many samples containing high levels of particulates were “filtered” by using an SPE step in the method. This method is easily amendable: simply replace the SPE step with a rapid and lower cost eXtreme|FV®step.

Applications for Thomson eXtreme|FV® include filtration of cell and cell debris from cell culture; pesticide analysis in food, tissue, soil, and water; and toxicology analysis.

page/fv-logo-ex3d.png

Multi-Mode Filtration

Max Fill Vol. 630µ
Dead Vol. 420µl

Thomson eXtractor3D|FV® Filter Vials offer filtration with increased volume enabling multiple extraction techniques with different resins/sorbents or solids/large particulates (greater than 35%) to autosampler ready vials. eXtractor3D|FV® is a product uniquely designed for the addition of resins/sorbents, QuEChERS dispersive salts, pills, or special resins in the standard autosampler ready vial.

Large solids/large particulates can be placed within the eXtractor 3D® where multiple extraction techniques occur. Prior to the introduction of the eXtractor3D|FV®, samples required multiple steps using SPE, or other methods to remove interfering analytes and co-eluting compounds. SPE or Quechers can now be completed with multi-depth filtration without risk of solids compromising the autosampler. Pills and other large solids can be broken down for complete testing using the eXtractor3D|FV®. eXtractor3D|FV® allows for compounds to be separated from the matrix with the addition of resins/sorbents, resulting in both a higher signal-to-noise ratio and greater peak differentiation.


What do Filter Vials Replace in the Lab?

Thomson Filter Vials simplify general filtration by replacing syringes & syringe filters, microcentrifuge spin columns, and/or liquid-liquid extractions.

Applications for Thomson Filter Vials include all sample types to be analyzed by HPLC, UHPLC, LC-MS, and GC-MS.

Filter Vials Can Optimize your SPE, D-SPE or QuEChERS Workflow

Thomson eXtractor3D|FV® has a shorter plunger allowing the addition of salts such as MgSO4 and or sorbents like C-18 to the shell vial eliminating the need for SPE columns and dispersive spin columns. This makes the eXtractor3D|FV® extremely versatile in any dispersive or SPE process.

Using eXtractor3D|FV® in Dispersive SPE

See figures to right

  1. Prepare Shell Vial
  2. Add Dispersive Salts (MgSO4) and/or Column Sorbents (C-18)
  3. Add Homonegized Sample
  4. Add Solvent / Buffer & Water to bring up to volume
  5. Vortex
  6. Depress Plunger completely to filter
  7. Prepared clean samples are now ready for LC/MS with separated salts, sorbents & sample debris

Plasticizers Content in Filter Vials
Compared to Syringe Filters

Introduction

Testing by Takeda Pharmaceutical Company Limited® UPLC – ELSD

Thomson Filter Vials are manufactured without the use of plasticizers or mold release agents, making them LC/MS clean. Testing with ELSD, PDA, and MS detection by Takeda Pharmaceutical showed no leaching from Thomson Standard Filter Vial with a 0.45um, PTFE membrane compared to signicant leaching from Millipore Millex-FH® Filter, 0.45M, hydrophobic PTFE, 4mm. Method: A. Water B. ACN 45-90% with .05% TFA Ballistic Gradient over 1.4 minutes using Waters® Acquity® UPLC Thomson Filter Vial (patented) Part # 35540-500 Filter Vial 0.45µM hydrophobic PTFE, w/ Pre-Slit Cap Millipore Syringe Filter Part #:SLFHR04NL Millex-FH® Filter, 0.45M, hydrophobic PTFE, 4mm, non-sterile.

Method

A. Water
B. ACN 45-90% with 0.05% TFA

Ballistic Gradient over 1.4 minutes using Waters® Acquity® UPLC

Thomson Standard Filter Vial

0.45µm hydrophobic PTFE, w/ Pre-Slit Cap
Part #: 35540-500

Millipore Syringe Filter

Millex-FH® Filter, 0.45µm, hydrophobic PTFE, 4mm, non-sterile.
Part #: SLFHR04NL

Plasticizers


Reduce Your Signal-to-Noise Ratio with eXtractor3D|FV®
For more targeted & accurate peaks

Matrix Effects & Ion Suppression:

Analytes are obscured by the matrix like the octopus in this photo is difficult to find among its surroundings.

page/fv-octo-1.jpg

Strong Signal; Noise Lessened:

By adding compounds to the eXtractor3D|FV® the signal to noise ratio is increased allowing you to find the analyte with ease.

page/fv-octo-2.jpg

Low Signal to Noise Ratio

Difficult to find analyte in the matrix

page/fv-matrix-full.jpg

High Signal to Noise Ratio

In this example the addition of C-18 to eXtraxtor3D|FV® with your sample binds excess compounds to C-18 and the Matrix clears up allowing you to see analyte peaks

page/fv-matrix-blank.jpg

High Viscosity Presses

The Thomson Filter Vial Press enables high solid content and viscous liquids to be easily filtered through vials. Some fermentation cultures that reach 100OD or particulate laden samples may require the toggle press.

Accessory Part Numbers

  • pn# {{ pn.num }} | case/qty: {{ pn.qty }}
  • pn# | case/qty: