Improved Method for the Analysis of a Pain Management Supplemental Panel in Urine using the Thomson eXtreme|FV® by LC-MS/MS

Poster presented at ASMS 2015
Nadine Koenig1, Crystal Xander1, Melanie Stauffer1, Dean Fritch2, Lisa Wanders3, Sam Ellis3
1Health Network Laboratories, 794 Roble Road, Allentown, PA 18109
2Analytical Associates, 225 Millwood Drive, East Greenville, PA
3Thomson Instrument Company, 1121 South Cleveland Street Oceanside, CA 92054

This improved sample preparation method allows for the quantitative measurement of the following pain management drugs in urine. The urine samples were diluted and filtered using Thompson eXtreme|FV®, followed by LC/MS/MS analysis. The most critical aspects of reliable urine analysis are the reduction of interferences from the sample matrix and analyte recovery. Traditionally, SPE, SLE and centrifugation have been used to reduce matrix interference prior to MS analysis. However, these techniques are time consuming, adversely impact recovery, require expensive consumables, lab equipment and use large amounts of solvent. Thomson eXtreme|FV® (patented) offer multi-layer filtration for viscous samples and samples containing up to 30% solid particulates. The filter vial consists of two parts: a filter vial outer shell and a plunger, which includes the multi-layer filter on one end and a vial cap on the other end.

Related Application Notes

Improved Method for the Analysis of a Pain Management Supplemental Panel in Urine using the Thomson eXtreme|FV® by LC-MS/MS

Traditionally, SPE, SLE and centrifugation have been used to reduce matrix interference prior to MS analysis. ... eXt...

See Application Note

Improved Sample Preparation Methods for Athlete Doping Analysis of Common Compounds in Urine by LCMS

The Australian Sports Drug Testing Laboratory, ... invested time in determining a limited number of comprehensive scree...

See Application Note

Automated Hydrolysis and Sample Preparation for the Analysis of 12 Opiates in Urine using the Thomson eXtreme Filter Vials® by LC-MS/MS

This improved sample preparation method allows for the quantitative measurement of Opioids in urine. Opioids are highly...

See Application Note

Clinical Urine Mega Method by LC-MS/MS

This improved sample preparation method allows for the quantitative measurement of over 60 drugs of different classes i...

See Application Note

Title

Improved Method for the Analysis of a Pain Management Supplemental Panel in Urine using the Thomson eXtreme|FV® by LC-MS/MS

Description

This improved sample preparation method allows for the quantitative measurement of the following pain management drugs in urine. The urine samples were diluted and filtered using Thompson eXtreme|FV®, followed by LC/MS/MS analysis. The most critical aspects of reliable urine analysis are the reduction of interferences from the sample matrix and an...

PDF Download

Products Used

eXtreme|FV® - PVDF 0.2µm

Pre-Split Septum, Red Cap

pn#85531-200

Multi-Use Press

48 Position for Autosampler Ready Filter Vials

pn#35015

Related Products

© 2021 Thomson |
Solutions At Work™

LinkedIn