Title
Evaluating the Impact of High Pluronic® F68 Concentrations on Antibody Producing CHO Cell Lines
Description
Routine analysis of samples containing cell culture media and/or supernatant by HPLC can lead to performance issues and can require frequent purchasing of new columns and increased HPLC maintenance. ... Here, we demonstrate that using Thomson [filter vials]] during sample preparation results in minimal impact preparation time, no detectable loss i...
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Evaluating the Impact of High Pluronic® F68 Concentrations on Antibody Producing CHO Cell Lines
Cell Science & Technology, Process & Product Development, Amgen Inc. One Amgen
Center Drive, Thousand Oaks 91320, California; telephone: 8054475321;
fax: 805-499-6819; e-mail: cgoudar@amgen.com
Routine analysis of samples containing cell culture media and/or supernatant by HPLC can lead to performance issues and can require frequent purchasing of new columns and increased HPLC maintenance. ... Here, we demonstrate that using Thomson [filter vials]] during sample preparation results in minimal impact preparation time, no detectable loss in product, and improved column performance leading to extended column lifetimes.