The bioprocess community is under a lot of pressure to shorten the timeframe from shake flask, to culture bag. Innovative solutions for aseptic sampling and feeding cultures in shake flasks as well as transferring from shake flasks to culture bags will be investigated. These solutions include flasks with integrated one-way valves that allow for sterile sampling outside of the tissue culture hood and shake flasks with bi-directional ports. The ports allow for coupling of sterile tubing that can aseptically transfer cells/media into and out of the flasks. These ports allow scientists to process samples in an aseptic fashion while the flasks remain in the incubator while maintaining the ambient temperature and eliminating disruptions in cell growth. These Optimum Growth® Flasks have the capability of speeding up and eliminating steps in bioprocess pipelines such as using, transferring material into and out of a cell bank, simplifying perfusion, and allowing for feeding of serum and other nutrients over time. Thomson Instrument Company strives to create tools that eliminate bottlenecks in biological process development