Screening and Quantitation of 250 Pesticides in Fruit Juices with Positive/Negative Switching LC/MS/MS

Poster presented at Florida Pesticide Residue Workshop 2014 by Bruker (CAM)
Zicheng Yang and Louis Maljers-Bruker, Chemical & Applied Markets Division, Fremont, CA

Liquid chromatography coupled with tandem mass spectrometry operated in multiple reaction monitoring (MRM) mode with electrospray ionization (ESI) is widely used for polar, semivolatile, and thermally labile pesticides in food testing. Many contract labs currently perform multi-residue analysis of pesticides using separate positive and negative methods due to instrument limitations especially for methods with hundreds of MRM transitions. This requires twice the sample and twice the analysis time. Recently, the Bruker EVOQ Elite LC-triple quadrupole system has been introduced to the market; thereby providing fast positive/negative switching allowing for simultaneous determination of positive and negative co-eluting compounds numbering in the hundreds. A study using the EVOQ analyzed 250 pesticides in apple juice, cranberry juice, grape juice, orange juice and vegetable juice using only one method with positive negative switching for over 500 MRM transitions. The measurements were conducted by dilute-and-shoot without sample enrichment. The fruit juices were diluted 10-fold and filtered by filter vial prior to injection. An YMC-Pack ODS-AQ, 3 µm, 150 mm x 3 mm (I.D.) column with mobile phases (A) 5 mM ammonium fluoride in water, and (B) methanol were used. The total run time was 18 minutes including reequilibration.

Related Application Notes

Screening and Quantitation of 250 Pesticides in Apple, Cranberry, Orange, Vegetable and White Grape Juices using the eXtreme|FV® by LC/MS/MS

[A]nalysis of 250 pesticides in store-bought juice using one method and simple sample preparation using the Thomson [eX...

See Application Note

Routine Targeted Quantitation & Identification of Pesticide Residues in Avocado, Carrot, Grape & Orange using the eXtreme|FV® by LC-MS/MS

Thomson eXtreme|FV®s for clean-up, and the Sciex Scheduled MRM Pro Algorithm for identification of pesticides in fruit ...

See Application Note

Tea Analysis with eXtreme|FV® by GC-MS

This method investigates whether SPE is required for the analysis of pesticides in green tea leaves using GC-MS.

See Application Note

Screening and Quantitation of 200+ Pesticides in Honey by an Integrated On-Line Extraction UHPLC-MS/MS System

[A] simple, cost effective and sensitive procedure for screening and quantitation of pesticides in honey using the Thom...

See Application Note

Analysis of Antibiotics in Honey by an Integrated On-Line Extraction UHPLC-MS/MS System

The most critical aspects of reliable food contamination analysis are the reduction of interferences from the sample ma...

See Application Note

eXtreme|FV® vs SPE for the Analysis of Pesticides in Orange Juice by GC/MS

Pesticides act as toxins when found in sufficient quantities as residues in food. Solid Phase Extraction (SPE) is a com...

See Application Note

Title

Screening and Quantitation of 250 Pesticides in Fruit Juices with Positive/Negative Switching LC/MS/MS

Description

Liquid chromatography coupled with tandem mass spectrometry operated in multiple reaction monitoring (MRM) mode with electrospray ionization (ESI) is widely used for polar, semivolatile, and thermally labile pesticides in food testing. Many contract labs currently perform multi-residue analysis of pesticides using separate positive and negative me...

PDF Download

Product Used

eXtreme|FV® - PVDF 0.2µm

Pre-Split Septum, Red Cap

pn#85531-200

Related Products

© 2021 Thomson |
Solutions At Work™

LinkedIn