Title
Optimum Growth® Transfer Caps Fast Aseptic Sterile Transfer of Fluids for Bioprocess from Flasks to Bags or Bioreactors
Description
Fast Aseptic Sterile Transfer of Fluids for Bioprocess from Flasks to Bags or Bioreactors Optimum Growth® Transfer Caps (patented) from Thomson Instrument Company when used with the Optimum Growth® Flasks allows your culture flask to double as your transfer flask, minimizing vessels. The Transfer Cap enables aseptic transfer of cells to your scale...
Products Used
Inversion Transfer Cap for Optimum Growth® 5L Flask
2' of C-Flex® 24 (O.D. 7/16") w/ Plug | Sterile
pn#931598-4
Optimum Growth® 5L Flask
PTFE 0.2µm Vent Cap for Increased Aeration | Sterile
pn#931116
Related Products
125mL 8 Position Carrier
Use with Optimum Growth® 125mL Flask (931110)
pn#1212900
Optimum Growth® 125mL Flask
PTFE 0.2µm Vent Cap for Increased Aeration | Sterile
pn#931110
Multiported Optimum Growth® 125mL Flask
PTFE 0.2µm Vent Cap for Increased Aeration | Sterile
pn#931110-DP
Rapid Clear® Cap 3000
High-speed clarification of 2L–4L of cell culture | Sterile
pn#788116
Optimum Growth® Transfer Caps Fast Aseptic Sterile Transfer of Fluids for Bioprocess from Flasks to Bags or Bioreactors
Julie Olson, Lisa Wanders, Joe Machamer, Julie Gallagher, Sam Ellis, Thomson Instrument Company, Oceanside, CA
Fast Aseptic Sterile Transfer of Fluids for Bioprocess from Flasks to Bags or Bioreactors Optimum Growth® Transfer Caps (patented) from Thomson Instrument Company when used with the Optimum Growth® Flasks allows your culture flask to double as your transfer flask, minimizing vessels. The Transfer Cap enables aseptic transfer of cells to your scale up vessel (Cell Bag or Bioreactor) inside or outside of the hood. The Optimum Growth® Transfer Cap system can use either gravity or pumping to facilitate transfer. The Inversion Caps are gravity powered and eliminate the need for pumping. Gravity transfer is much gentler on the cells and maintains higher culture viability than when cells are pumped. Flow rates for the 7/16” connection are on average 1L/min, while for the 1/4” connection the flow is around 0.5L/min. The Bidirectional Caps have a down-stem and require a peristaltic pump. This allows for more accurate control when transferring to multiple vessels. It enables media to be pumped into the flasks from a bulk source. Transfer caps can be quick connected or tube fused depending on the caps used. Transfer Caps are compatible with all culture bag systems and bioreactors. The addition of the down stem tubing to the Optimum Growth® Transfer Cap System allows for the Optimum Growth® Flasks to be used with a peristaltic pump. This design enables very precise control of the volume transferred to one or many vessels for inoculation from a single seed culture. The Pump Transfer Cap is bidirectional, so liquid can be pumped both in and out of the Optimum Growth® Flasks.
Related Application Notes
Optimum Growth® Protocol for Insect Cells
... Sf9 and Sf21 insect cells are cell lines commonly used for expression of recombinant proteins using baculovirus sys...
See Application NoteSimplified Sample Prep for Open Access SEC-HPLC Detection using the Thomson Filter Vials
Routine analysis of samples containing cell culture media and/or supernatant by HPLC can lead to performance issues and...
See Application NoteComparison of the Rapid Clear® Cap 3000 (patented) vs GE Capsule Filters for clarifying monospecific IgG
Two methods to clarify 10L of post transfected IgG harvested from culture are compared. The culture was harvested on da...
See Application NoteImproved DNA Protocol for E.coli with Plasmid+® Media
Plasmid+® liquid media is an enriched media specifically designed for plasmid DNA production. Plasmid+® supports much h...
See Application Note