Optimum Growth™ Transfer Caps Fast Aseptic Sterile Transfer of Fluids for Bioprocess from Flasks to Bags or Bioreactors

Poster presented at PepTalk 2015
Julie Olson, Lisa Wanders, Joe Machamer, Julie Gallagher, Sam Ellis, Thomson Instrument Company, Oceanside, CA

Fast Aseptic Sterile Transfer of Fluids for Bioprocess from Flasks to Bags or Bioreactors Optimum Growth™ Transfer Caps (patented) from Thomson Instrument Company when used with the Optimum Growth™ Flasks allows your culture flask to double as your transfer flask, minimizing vessels. The Transfer Cap enables aseptic transfer of cells to your scale up vessel (Cell Bag or Bioreactor) inside or outside of the hood. The Optimum Growth™ Transfer Cap system can use either gravity or pumping to facilitate transfer. The Inversion Caps are gravity powered and eliminate the need for pumping. Gravity transfer is much gentler on the cells and maintains higher culture viability than when cells are pumped. Flow rates for the 7/16” connection are on average 1L/min, while for the 1/4” connection the flow is around 0.5L/min. The Bidirectional Caps have a down-stem and require a peristaltic pump. This allows for more accurate control when transferring to multiple vessels. It enables media to be pumped into the flasks from a bulk source. Transfer caps can be quick connected or tube fused depending on the caps used. Transfer Caps are compatible with all culture bag systems and bioreactors. The addition of the down stem tubing to the Optimum Growth™ Transfer Cap System allows for the Optimum Growth™ Flasks to be used with a peristaltic pump. This design enables very precise control of the volume transferred to one or many vessels for inoculation from a single seed culture. The Pump Transfer Cap is bidirectional, so liquid can be pumped both in and out of the Optimum Growth™ Flasks.

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Title

Optimum Growth™ Transfer Caps Fast Aseptic Sterile Transfer of Fluids for Bioprocess from Flasks to Bags or Bioreactors

Description

Fast Aseptic Sterile Transfer of Fluids for Bioprocess from Flasks to Bags or Bioreactors Optimum Growth™ Transfer Caps (patented) from Thomson Instrument Company when used with the Optimum Growth™ Flasks allows your culture flask to double as your transfer flask, minimizing vessels. The Transfer Cap enables aseptic transfer of cells to your scale...

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Products Used

Inversion Transfer Cap for Optimum Growth™ 5L Flask

2' of C-Flex® 24 (O.D. 7/16") w/ Plug | Sterile

pn#931598-4

Optimum Growth™ 5L Flask

PTFE 0.2µm Vent Cap for Increased Aeration | Sterile

pn#931116

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