New Invention: Harvesting CHO and HEK293 Cells with the Rapid Clear® Cap provides 0.2μm sterile filtration in a fraction of the time required by traditional clarification methods

Poster presented at PepTalk 2017 on the Rapid Clear® Cap
Author: Sam Ellis3, Eric Ailor1, Charles Dillard2, Ana Sirianni3, Lisa Wanders3
1Mapp Biopharmaceutical, Inc., San Diego, CA; 2Dart NueroScience, San Diego, CA; 3Thomson Instrument Company, Oceanside, CA

The Thomson Optimum Growth™ Family of products is expanding into downstream processing with a revolutionary new technology that allows high speed clarification of cellular material. Simply switch the standard vented Optimum Growth™ Cap to the Rapid Clear® Cap (patented). The only equipment required is a peristaltic pump. In initial testing, the Rapid Clear® Cap can filter up to 2.5L-3L of high density culture [6x10^6 -20x10^6 @ >70% viability] in approximately 18 minutes. At lower or higher densities and viabilities the total volume and processing speeds will differ. This technique will transform the time consuming and laborious process of harvesting cells to a rapid, walk away procedure.

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Title

New Invention: Harvesting CHO and HEK293 Cells with the Rapid Clear® Cap provides 0.2μm sterile filtration in a fraction of the time required by traditional clarification methods

Description

The Thomson Optimum Growth™ Family of products is expanding into downstream processing with a revolutionary new technology that allows high speed clarification of cellular material. Simply switch the standard vented Optimum Growth™ Cap to the Rapid Clear® Cap (patented). The only equipment required is a peristaltic pump. In initial tes...

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Products Used

Rapid Clear® Cap 3000

High-speed clarification of 2L–4L of cell culture | Sterile

pn#788116

Optimum Growth™ 5L Flask

PTFE 0.2µm Vent Cap for Increased Aeration | Sterile

pn#931116

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