An LCMS Method for the Detection of Cocoa Butter Substitutes, Replacers, and Equivalents in Commercial Chocolate-like Products

no citations

There is increasing demand for genuine cocoa butter (CB) in chocolate products in developed nations, however, this demand has created a shortage of CB and raised its costs. To overcome this, chocolate manufactures sometimes add vegetable-derived fats to some chocolate products to reduce costs while still maintaining desirable physical characteristics. It is of current interest to have a reliable method to detect, identify, and quantify the triacylglycerol (TAG) components of cocoa butter substitutes, replacers, and equivalents (CBEs) in chocolate products. Traditionally GC was used for this task, but due to the low volatility of triacylglycerides and their susceptibility to thermal decomposition, retention time is the only identifying factor for the TAGs and typical GC analyses of this type can take 40 minutes. LCMS is able to not only provide faster throughput, but also has the additional advantage of allowing characterization of the TAG, including qualitative regiospecific analysis. We have developed a single, UHPLC column-based LCMS method to analyze the TAG components in commercial chocolate and chocolate-like products. This analysis has a runtime of 17minutes, making it suitable for relatively high throughput. Additionally, the method was very repeatable, with an interday variability of <7% for the absolute area counts of the three major TAGs in CB (POP,POS,SOS).

Related Application Notes

Analysis of Antibiotics in Honey by an Integrated On-Line Extraction UHPLC-MS/MS System

The most critical aspects of reliable food contamination analysis are the reduction of interferences from the sample ma...

See Application Note

Screening and Quantitation of 200+ Pesticides in Honey by an Integrated On-Line Extraction UHPLC-MS/MS System

[A] simple, cost effective and sensitive procedure for screening and quantitation of pesticides in honey using the Thom...

See Application Note

eXtreme|FV® vs SPE for the Analysis of Pesticides in Orange Juice by GC/MS

Pesticides act as toxins when found in sufficient quantities as residues in food. Solid Phase Extraction (SPE) is a com...

See Application Note

Screening and Quantitation of 250 Pesticides in Apple, Cranberry, Orange, Vegetable and White Grape Juices using the eXtreme|FV® by LC/MS/MS

[A]nalysis of 250 pesticides in store-bought juice using one method and simple sample preparation using the Thomson [eX...

See Application Note

Routine Targeted Quantitation & Identification of Pesticide Residues in Avocado, Carrot, Grape & Orange using the eXtreme|FV® by LC-MS/MS

Thomson eXtreme|FV®s for clean-up, and the Sciex Scheduled MRM Pro Algorithm for identification of pesticides in fruit ...

See Application Note

Tea Analysis with eXtreme|FV® by GC-MS

This method investigates whether SPE is required for the analysis of pesticides in green tea leaves using GC-MS.

See Application Note

Title

An LCMS Method for the Detection of Cocoa Butter Substitutes, Replacers, and Equivalents in Commercial Chocolate-like Products

Description

There is increasing demand for genuine cocoa butter (CB) in chocolate products in developed nations, however, this demand has created a shortage of CB and raised its costs. To overcome this, chocolate manufactures sometimes add vegetable-derived fats to some chocolate products to reduce costs while still maintaining desirable physical characterist...

PDF Download

Product Used

Standard|Filter Vial - Nylon 0.2µm

Pre-Split Septum, Black Cap

pn#35538-200

Related Products

© 2021 Thomson |
Solutions At Work™

LinkedIn