Title
Scalable Results 30mL-2L in Optimum Growth® Flasks with ExpiCHO™ Expression System after Transfection
Description
The Thomson Optimum Growth® line of flasks provides robust growth and protein expression of CHO suspension cells and their derivatives. One of the most popular lines is the ExpiCHO™ Expression System from Thermo Fisher Scientific. Although a proven workhorse for mABs and antigen expression, difficulty in scaling up beyond 500mL has been ...
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Scalable Results 30mL-2L in Optimum Growth® Flasks with ExpiCHO™ Expression System after Transfection
Dominic Esposito, Director, Protein Expression Laboratory at Frederick National Laboratory for Cancer Research
The Thomson Optimum Growth® line of flasks provides robust growth and protein expression of CHO suspension cells and their derivatives. One of the most popular lines is the ExpiCHO™ Expression System from Thermo Fisher Scientific. Although a proven workhorse for mABs and antigen expression, difficulty in scaling up beyond 500mL has been a limitation of the system. Below we present a method for scaling up to 2L, thereby improving efficiency in the workflow and economy of space.