Saliva test is one of the easiest, cost-effective and most accurate ways to measure the presence of drugs in the body. Collecting saliva sample is relatively non-invasive, easier to procure and reduced risk of sample adulteration. However, saliva matrix display much lower levels of drug compounds compared to urine samples, making the need to test at lower cut-off levels more important. ... Here we present a high throughput, cost effective and sensitive procedure for screening and confirmation of Pain Panel Drugs (PPDs) in Synthetic Saliva using Thomson filter vial for sample preparation and using an integrated On-Line Extraction (OLE)-UHPLC-MS/MS System for sample analysis.