Title
Protocol: Thawing and Seeding Mammalian or Insect Cells in Optimum Growth® Flasks
Description
General procedure for thawing cryopreserved mammalian or insect suspension cells into a 125mL or 250mL Thomson flask to a concentration of 0.5x106 cells/mL. Match post-thaw cell number to an appropriate flask working volume and shaker speed to maintain the desired seeding density and agitation environment during recovery.
Protocol: Thawing and Seeding Mammalian or Insect Cells in Optimum Growth® Flasks
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General procedure for thawing cryopreserved mammalian or insect suspension cells into a 125mL or 250mL Thomson flask to a concentration of 0.5x106 cells/mL. Match post-thaw cell number to an appropriate flask working volume and shaker speed to maintain the desired seeding density and agitation environment during recovery.
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