7L Flask
7L Flask is the gold standard for Bioprocessing that customers have been using for mammalian (5L) and insect (6L) cell lines.
Scale With Thomson 7L Flasks
The Bioprocessing Choice!
Addressing the limitations of large-scale culture methods
The Thomson Optimum Growth® 7L Flask increases productivity while minimizing space and consumable costs. This efficiency results in up to 35L of expression per shaker, making it an excellent alternative to bulky 10L, 20L, or 50L bags for CHO and HEK293 applications.
Comparable results
Cost efficient
Fits on existing platforms
Less complicated subparts
Learn More About 7L Flask
7L Flask Is Robotic Friendly!
7L Features
Features & Benefits
High Working Volume: Supports 5L for mammalian and hybridoma cells (CHO, HEK293) and up to 6L for insect cell lines (SF9, SF21) per flasks, delivering up to 35L of expression per shaker.
Scalability: Seamlessly scales from 125mL to 7L, enabling efficient transitions from small-scale experiments to large-scale production without compromising consistency.
Baffle Design: Proprietary baffles ensure high aeration with low shear, maintaining cell viability and maximizing protein yield.
0.2μm Vented Cap: Promotes high gas exchange while maintaining sterility, reducing contamination risks.
Transfer Options: Connects directly to bioreactors or cell bags with bidirectional transfer caps (Part #931470-8) for seamless scale-up.
Single-Use Convenience: Individually packaged and sterilized, ready for immediate use.
Scalable Results with Every Optimum Growth® Flask
7L Fill Volume & Shake Speeds For Mammalian, Hybridoma, Microbial & Insect Cell Lines
Working fill volume & shake speeds for Optimum Growth® 7L Flasks for the best advertised results.
| Cell Line | Fill Volume | Working RPM Range 1" (2.54cm) | 2" (5.08cm) | Vol./Size Ratio |
|---|---|---|---|
| Mammalian | 2.8-5L | 140-150 | 110 | 40-70% |
| Insect | Up to6L | 135 | NA | 85% |
Visit More of the Optimum Growth® System
Scalable Results for Expi® HEK293 with Optimum Growth® Flasks
7L Flasks Outperforms 5L Flasks
Data provided by Pfizer
Our partners at Pfizer evaluated Expi® HEK293 cell growth
Thomson's Optimum Growth® 5L Flask (2.5L working volume) andOptimum Growth® 7L Flask (5L working volume) deliver exceptional performance for cell culture scaling. Our partners at Pfizer evaluated Expi® HEK293 cell growth in both 5L and 7L Thomson volumes. The 7L flask (5L working volume) matched the performance of the 5L flask (2.5L working volume) while offering twice the capacity within the same footprint.
Thomson is not affiliated with Pfizer & ThermoFisher
high-aeration design ensures optimal conditions
Its high-aeration design ensures optimal conditions for daily cell doubling and high viability, preparing cells for downstream applications. The 7L flask adds greater versatility to the Optimum Growth® line, supporting flexible scaling options. All flasks were shaken at 125 rpm on a 0.75” (19mm) orbital shaker.
Viability (%)
| Flask ID | 0hrs | 24hrs | 48hrs | 72hrs |
|---|---|---|---|---|
| 2.5L Expi293 in 5L | 96.3 | 96.8 | 97.8 | 97.3 |
| 5L Expi293 in 7L | 97.2 | 97.2 | 96.5 | 98 |
Viable Cell Count at e6 cells/ml
| Flask ID | 0hrs | 24hrs | 48hrs | 72hrs |
|---|---|---|---|---|
| 2.5L Expi293 in 5L | 5.9 | 10.4 | 18.3 | 44.3 |
| 5L Expi293 in 7L | 6.3 | 11.3 | 17.5 | 41.6 |
Consistent IgG1 Expression Across Various Scales
Data provided by large pharma customer
2.5L(5L) & 5L(7L) Final volume
Thomson 7L flasks are ideal for scaling up protein production in transient CHO, stable CHO, HEK293, and insect cells, supporting final volumes up to 5L-6L. They ensure scalable protein quality and titer, as demonstrated by consistent IgG1 expression across various scales.
Thomson is not affiliated with MilliporeSigma
7L Flask Outperforms 200L Bioreactor
Data provided by Lundbeck
Lundbeck evaluated the 7L Flask against a 200L bioreactor
Our partners at Lundbeck utilized the 7L flask to rapidly scale eight antibody constructs and compared the results to a 200L bioreactor used for standard antibody production. The 7L flask achieved nearly identical yields to the bioreactor while maintaining higher cell density and viability, with lower lactate levels. Culture conditions were set at 150 rpm (25mm orbit), 6% CO2, and 80% humidity, showcasing the flask’s ability to deliver bioreactor-comparable performance in a compact, efficient system.
Thomson is not affiliated with Lundbeck
Data provided by Lundbeck.
Thomson is not affiliated with Lundbeck
7X 7L Allows for Large Culture Volumes As Replacement of 50L Wave Bags
Comparable Results to Wave Bags
Data provided by Lundbeck
Why are 7L flasks better than wave bags?
Thomson 7L flasks provide exceptional performance for high-volume cell culture production, matching the efficiency of larger 50 L cell culture bags while offering greater flexibility. By utilizing Thomson 7L flasks, researchers can achieve consistent, large-scale cell culture results, making them an ideal alternative for optimizing workflows in demanding bioprocessing environments.
Thomson is not affiliated with Lundbeck, Corning, or Cytiva
Up to 6L Protein Expression in SF21 Cells
Data provided by Pfizer
produce proteins with high accuracy & integrity
Mass Spec analysis of protein expressed in the Thomson Optimum Growth® 7L Flask confirms identity of the expression target. This precise measurement validates the flask's ability to produce proteins with high accuracy and integrity
Thomson is not affiliated with Pfizer
Intact LC/MS (Sequence MW: 61,245 Da)
Protein of Interest - Affinity Capture/Elution (IMAC)
LC/MS & SDS-PAGE Results
The LC/MS results show the Thomson 7L Flask's suitability for applications requiring rigorous protein characterization. Data shows the flask produces consistent, high-fidelity protein outputs for downstream analytical workflows.
The Thomson Optimum Growth® 7L Flask is one of the best solutions for high-volume cell culture, offering scalability, efficiency, and performance.
SDS-PAGE Reducing Gel
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